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纸质出版日期:2018
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赵停, 李静, 安衍茹, 等. 光质、光强对远志种子萌发和幼苗生理特性的影响[J]. 中国实验方剂学杂志, 2018,24(17):68-73.
ZHAO Ting, LI Jing, AN Yan-ru, et al. Effect of Light Quality and Intensity on Seed Germination and Seedling Physiological Characteristics of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 68-73.
赵停, 李静, 安衍茹, 等. 光质、光强对远志种子萌发和幼苗生理特性的影响[J]. 中国实验方剂学杂志, 2018,24(17):68-73. DOI: 10.13422/j.cnki.syfjx.20181010.
ZHAO Ting, LI Jing, AN Yan-ru, et al. Effect of Light Quality and Intensity on Seed Germination and Seedling Physiological Characteristics of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 68-73. DOI: 10.13422/j.cnki.syfjx.20181010.
目的:通过不同光质、光强对种子萌发、幼苗生理特性和总酚、总黄酮含量影响的研究,为远志的前期育苗提供参考,为增加远志中总酚、总黄酮的含量提供理论依据。方法:用不同的光质、光强干预远志种子的发芽和幼苗的生长,测定种子的发芽率、发芽势、发芽指数、活力指数、幼苗的株高、根长、干重、鲜重及总酚、总黄酮含量。结果:红光下远志种子的发芽指标及幼苗的生长指标均显著高于白光,白光下的幼苗总黄酮含量最高,红光下总酚含量最高。不同光照强度对远志种子的发芽率、发芽势、发芽指数无影响,300,500 μmol·m-2·s-1的光强促进远志幼苗的生长,100 μmol·m-2·s-1光强下总黄酮含量最高,300 μmol·m-2·s-1光强下总酚含量最高。结论:不同光质、光强对种子发芽和幼苗生长的影响表明,红光能够促进远志种子的萌发和远志幼苗的生长,白光促进总黄酮的生成,红光促进总酚的生成。光照强度对远志种子的萌发无影响,500 μmol·m-2·s-1的光照强度更适宜于远志幼苗的生长,100 μmol·m-2·s-1的光强有利于总黄酮的生成,300 μmol·m-2·s-1的光强有利于总酚的生成。研究表明,光质光强对远志幼苗生长和总酚总黄酮含量的影响,可能与远志种子及幼苗内的激素含量有关,但具体过程还有待进一步验证。
Objective:To provide a reference for early seedlingof Polygala tenuifolia and provide theoretical basis for increasing total phenols and total flavonoids through the study on the effects of light quality and intensity on seed germination
seedling physiological characteristics of P. tenuifolia as well as on total phenolics and total flavonoids. Method:Light with quality and intensity was used tointervene the germination of seed and the growth of seeding of P. tenuifolia
and then the germination rate
germination energy
germination index
vigor index
plant height
root length
dry weight
fresh weight
total phenolics and total flavonoids were detected and determined. Result:The germination index and seedling growth index of P. tenuifolia under red light were significantly higher than those of control group
and the total flavonoids content of seedlings under white light was highest
while the total phenols content was highest in red light.The germination rate
germination potential and germination index of seeds of P. tenuifolia were not affected by different light intensity.The light intensity of 300
500 μmol·m-2·s-1 promoted the growth of P. tenuifolia seedlings. The total flavonoids content under the light intensity of 100 μmol·m-2·s-1 was highest
and the total phenols content was highest under the light intensity of 300 μmol·m-2·s-1. Conclusion:The effects of different light quality and intensity on seed germination and seedling growth showed that red light can promote the germination of P. tenuifolia seeds and the growth of P. tenuifolia seedlings; white light could promote the formation of total flavonoids
and red light could promote the production of total phenolics. Light intensity had no effect on seed germination of P. tenuifolia.The illumination intensity of 500 μmol·m-2·s-1 was more suitable for the growth of P. tenuifolia seedlings.The light intensity of 100 μmol·m-2·s-1 was beneficial to the formation of total flavonoids
and the light intensity of 300 μmol·m-2·s-1 was beneficial to the formation of total phenols. Research showed that the effects of light quality and intensity on seedling growth and the content of total flavonoids and total phenolics of P. tenuifolia
may be related to hormones content
but the specific process remained to be verified.
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