Objective: To investigate the chemical constituents from the carpopodium of Schisandra chinensis for further research of its potential medicinal value

and provide certain chemical basis and foundation for the comprehensive development and the search for pharmacological activity. Method: The air-dried carpopodium of S. chinensis (6.0 kg) was extracted with 70% ethanol under reflux for 3 times

2 hours each time. After removal of solvent

the crude extract was suspended in H2O and further extracted with petroleum ether

ethyl acetate and n-butyl alcohol. The EtOAc fraction was further isolated and purified by using positive phase silica gel column chromatography

reversed phase ODS column chromatography

Sephadex LH-20 and preparative HPLC. The structures of the isolated compounds were determined by spectroscopic methods including1H-NMR and13 C-NMR as well as HR-ESI-MS data. Result: Ten monomeric compounds were isolated from the ethyl acetate fraction of the 70% ethanol extract from the carpopodium of S. chinensis

and were identified as 1β

4α

11α-trihydroxyeudesmane (1)

bullatantriol (2)

alismoxide (3)

sonnerstigmane D (4)

zataroside-A (5)

magnoliaterpenoid C (6)

(Z)-furanosyl-linalooloxide-7-O-[β-D-apiofura-nosyl- (1-6)-1-β-D-glucopyranoside] (7)

thymoquinol 2-O-β-D-apiofuranosyl- (1-6)-β-D-gluco-pyranoside (8)

8-hydroxy-neo-menthol (9)

and 9-hydroxy-megastigma-4

7-dien-3-one-9-O-β-D-glucopyranoside (10). Conclusion: Compounds 1-7

9

10 were isolated from the plants of Schisandra genus for the first time. The discovery of these compounds further enriched the chemical constituents of S. chinensis

providing experimental and scientific basis for the comprehensive development and utilization of S. chinensis.