Objective: To rapidly analyze the anti-complement active constituents in the Rheum ihasaenseby UPLC-Q-TOF-MS/MS technology

and to provide a reference for elucidating the anti-complement activity pharmacological basis of the rhubarb. Method: 95% ethanol extract of rhubarb was extracted to obtain petroleum ether

ethyl acetate and n-butanol. The anti-complement hemolytic activity (CH50) of three parts was determined. UPLC-Q-TOF-MS/MS was used to characterize the chemical constituents of the three parts by using the target compound screening method in the mass spectrometry software

and the chemical composition was identified by the retention time

the exact molecular weight and the secondary mass spectrometry. Result: The values of anticomplement activity of adipsoin was 0.032·mL-1

which was stronger than that of positive drug heparin. In the optimized LC-MS

the scifinder database

reference substance and related literature werecombined to identify compounds. The main components of petroleum ether were identified

which were mainly fatty acids and sterols. Thirteencompounds from ethyl acetate were identified

mainly free anthraquinones and stilbenes. The main components of n-butanol were identified as mainly anthraquinone glycosides and stilbene glycosides. Conclusion: Based on UPLC-Q-TOF-MS/MS technology

the chemical constituents of the n-butanol-resistant part of the reaction group were mainly anthraquinone and stilbene glycosides

which provided foundation for furtherseparation and development of anti-complement active ingredients from rhubarb.