白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响

赵欣; 恽海峰; 向桂玲; 韩丹; 金逸纯; 孙宏文; 姚瑶

doi:10.13422/j.cnki.syfjx.20181037

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白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响

药理 | 更新时间：2024-01-04

- 白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响
- Effect and Mechanism of Modified Baitouweng Tang Combined with Bifidobacterium Capsule on Rat Model of Ulcerative Colitis
- 中国实验方剂学杂志 2018年24卷第13期页码：110-116
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药管理局吴门医派杂病流派传承工作室项目（[2012]228）;苏州市科技局民生科技-医疗卫生应用基础研究[第五项]项目（SYSD2016192）
- DOI：10.13422/j.cnki.syfjx.20181037
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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赵欣, 恽海峰, 向桂玲, 等. 白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响[J]. 中国实验方剂学杂志, 2018,24(13):110-116.

ZHAO Xin, YUN Hai-feng, XIANG Gui-ling, et al. Effect and Mechanism of Modified Baitouweng Tang Combined with Bifidobacterium Capsule on Rat Model of Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 110-116.
赵欣, 恽海峰, 向桂玲, 等. 白头翁汤加减联合双歧杆菌制剂对大鼠溃疡性结肠炎的影响[J]. 中国实验方剂学杂志, 2018,24(13):110-116. DOI： 10.13422/j.cnki.syfjx.20181037.

ZHAO Xin, YUN Hai-feng, XIANG Gui-ling, et al. Effect and Mechanism of Modified Baitouweng Tang Combined with Bifidobacterium Capsule on Rat Model of Ulcerative Colitis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 110-116. DOI： 10.13422/j.cnki.syfjx.20181037.

摘要

目的：探讨白头翁汤加减联合双歧杆菌制剂治疗溃疡性结肠炎的效果及其作用机制研究。方法：SPF级SD大鼠60只，随机抽取8只大鼠作为正常组，剩余52只造模。采用2，4，6-三硝基苯磺酸（TNBS）/乙醇灌肠法复制溃疡性结肠炎模型，第3天随机抽取2只解剖，观察造模是否成功。剩余的50只大鼠随机分为5组，每组10只，分别为模型组，丽珠肠乐（双歧杆菌制剂）组（丽珠肠乐组），丽珠肠乐+白头翁汤加减高、中、低浓度组（联合高、中、低剂量组），定时给药持续14 d，评估大鼠疾病活动指数（diseaseactivityindex，DAI）。取材后行苏木素-伊红（HE）染色；酶联免疫吸附测定法（ELISA）检测血清D-乳酸（D-lactic acidosis，D-LA），二胺氧化酶（double amine oxidase，DAO）水平及结肠细胞因子白细胞介素-8（IL-8），IL-13水平；实时荧光定量-聚合酶链式反应（Real-time PCR）法检测双歧杆菌及IL-1β mRNA的表达。结果：与正常组比较，模型组大鼠体质量明显降低，DAI和HE染色评分均明显升高，血清D-LA，DAO及结肠组织IL-8水平明显升高，IL-13水平明显降低，双歧杆菌mRNA明显降低及IL-1βmRNA明显升高（P<0.05）；联合组高剂量组死亡率低于丽珠肠乐组，各给药组大鼠体质量均高于模型组（P<0.05）；联合组高剂量组死亡率低丽珠肠乐组，且体质量高于丽珠肠乐组（P<0.05）；联合组高剂量组DAI低于丽珠肠乐组（P<0.05）；联合组高剂量组HE评分低于丽珠肠乐组（P<0.05）；与丽珠肠乐组比较，联合组高剂量组D-LA，DAO和IL-8降低，IL-13升高（P<0.05）；各用药组大鼠结肠组织中双歧杆菌mRNA相对含量均有增加（P<0.05）；联合组高剂量组双歧杆菌mRNA相对含量高于丽珠肠乐组（P<0.05）；联合组高剂量组IL-1β的相对表达量低于丽珠肠乐组（P<0.05）。结论：高剂量白头翁汤加减联合双歧杆菌制剂可能通过促进溃疡性结肠炎大鼠结肠黏膜修复，加强肠道双歧杆菌定植，降低D-LA，DAO和IL-8，升高IL-13水平，下调IL-1β mRNA表达发挥协同治疗作用。

Abstract

Objective: To investigate the effect and mechanism of modified Baitouweng Tang combined with bifidobacterium capsule in the treatment of ulcerative colitis. Method: Eight of a total of 60 SD rats were randomly selected as normal group(group A). We replicated the ulcerative colitis model with the remaining 52 rats by TNBS/ethanol enema. Rats were dissected at random at the 3rd days

so as to observe if the model was successfully established. The remaining 50 rats were randomly divided into 5 groups:modern group(group B)

bifidobiogen (bifidobacterium capsule)group(group C)

and high

medium and low-concentration bifidobiogen+modified Baitouweng Tang of group(group D

with 10 rats in each group. After 2 weeks of treatment

disease activity index (DAI) in rats was evaluated. HE staining was performed after the materials were collected. ELISA was used to detect serum D-lactate (D-LA)

two amine oxidase (DAO) levels and colon tissue cytokine IL-8 and IL-13 levels. Real-time PCR method was used to detect the mRNA expressions of Bifidobacterium and IL-1β. Result: The mortality of group D was lower than that in group C. The weight of each drug group was higher than that of group B (P<0.05); group D had a lower mortality than group C

and the weight was higher than that of group C (P<0.05); DAI in group D was lower than that of group C (P<0.05). HE scale in D group was lower than that of group C (P<0.05); compared with group C

group D had lower D-LA

DAO and IL-8 levels and a higher IL-13 level (P<0.05).Bifidobacterium mRNA relative content in group D was higher than that of group C (P<0.05); the expression of IL-1β in group D was lower than that of group C (P<0.05). Conclusion: High concentration of modified Baitouweng Tang combined with bifidobacterium capsule may play a synergistic role in promoting colonic mucosal repair

strengthening bifidobacterium colonization in intestinal tract

reducing D-LA

DAO and IL-8

increasing IL-13 level

and down-regulating the mRNA expression of IL-1β in ulcerative colitis rats.

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