Objective: To isolate and identify Bletillae Rhizoma fungi and study on the exopolysaccharides activity of endophytic fungi

in order to alleviate the pressure of Bletillae Rhizoma medicinal resources. Method: Bletillae Rhizoma endophytic fungi were isolated and identified

and then exopolysaccharides were obtained through liquid fermentation. The antioxidant activity of exopolysaccharide in Bletillae Rhizoma roots endophytic fungi was studied by establishing 4 kinds of in vitro models

including iron potassium cyanide

2

2-diphenyl-1-picrylhydrazyl (DPPH)

salicylic acid and 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Result: The 10 endophytic fungi were isolated from Bletillae Rhizoma fresh roots

and 6 strains were obtained after identification and combination. The exopolysaccharides of No.10 strain (0.1 g L-1) had the strongest total reducing power

with VC equivalent up to 12.01 mg·L-1

followed by No. 6 strain. No. 1 and No. 6 strains had the strongest DPPH free radical scavenging activities (16.6%). Strain 6 had the strongest hydroxyl radical scavenging activities (21.6%) and No.10 strain had the weakest activity (11.0%) on scavenging hydroxyl radical. In the detection of ABTS free radical scavenging activity

it was found that No. 10 strain had the strongest activity (13.6%)

followed by No. 6 strain

and the weakest was only 1.8% for No. 5 strain. Conclusion: The exopolysaccharides of Bletillae Rhizoma endophytic fungi has certain antioxidant activity

and the exopolysaccharides of No. 6 strain has better total reducing power

higher scavenging activities of DPPH free radicals

hydroxyl radicals

and ABTS free radicals

so it can be further developed and utilized.