Objective: To study the protective mechanism of Chaijie Yiganxian (CJYGX) on the liver fibrosis through hepatic stellate cells (HSC-T6) and transforming growth factor-β1/signal transduction protein (TGF-β1/Smad) signaling pathway. Method: Different concentrations of colchicine (1.0 mg·L-1) and CJYGX (200

100

50

25

12.5

6.25 mg·L-1) were added to the culture medium

and the proliferation of HSC-T6 was detected by cell counting kit-8 (CCK-8) after 24 hours. The appropriate drug concentration that can inhibit the cell proliferation was screened out according to the CCK-8 results. The mRNA expressions of TGF-β1

Smad2

Smad3

Smad4 and Smad7 in HSC-T6 cells treated with CJYGX (200

100

50 mg·L-1) were detected by Real-time PCR

and the protein expressions of TGF-β1 and α-SMA also were detected by Western blot. Result: Compared with the blank group

CJYGX (200

100

50 mg·L-1) had a significantly inhibitory effect on HSC-T6 cells (P<0.05).CJYGX (200

100

50 mg·L-1) can significantly down-regulate the mRNA expressions of TGF-β1

Smad2 and Smad3 (P<0.05). Meanwhile

CJYGX (200

100 mg·L-1) can significantly down-regulate Smad4 mRNA expression and up-regulated Smad7 mRNA expression (P<0.05).CJYGX (200

100 mg·L-1) can significantly down-regulate the protein expressions of TGF-β1 and α-SMA in HSC-T6 cells(P<0.05). Conclusion: CJYGX can inhibit HSC-T6 cell proliferation

and its mechanism is related to TGF-β1/Smad signaling pathway.