四君子汤加减对脑缺血/再灌注损伤大鼠脑组织ERK1/2，Akt，Bax表达的影响

胡康丽; 李花; 刘旺华; 何倩; 钟银玲; 彭智远

doi:10.13422/j.cnki.syfjx.20181092

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四君子汤加减对脑缺血/再灌注损伤大鼠脑组织ERK1/2，Akt，Bax表达的影响

药理 | 更新时间：2024-01-04

- 四君子汤加减对脑缺血/再灌注损伤大鼠脑组织ERK1/2，Akt，Bax表达的影响
- Effect of Modified Si Junzitang on Expression of ERK1/2, Akt, Bax in Rats with Cerebral Ischemia/Reperfusion Injury
- 中国实验方剂学杂志 2018年24卷第13期页码：152-158
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81202632，81473567）;湖南省教育厅优秀青年基金项目（14B134）;湖南省高校创新平台基金项目（15K092）;湖南省中医药科研计划项目（201723）
- DOI：10.13422/j.cnki.syfjx.20181092
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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胡康丽, 李花, 刘旺华, 等. 四君子汤加减对脑缺血/再灌注损伤大鼠脑组织ERK1/2，Akt，Bax表达的影响[J]. 中国实验方剂学杂志, 2018,24(13):152-158.

HU Kang-li, LI Hua, LIU Wang-hua, et al. Effect of Modified Si Junzitang on Expression of ERK1/2, Akt, Bax in Rats with Cerebral Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 152-158.
胡康丽, 李花, 刘旺华, 等. 四君子汤加减对脑缺血/再灌注损伤大鼠脑组织ERK1/2，Akt，Bax表达的影响[J]. 中国实验方剂学杂志, 2018,24(13):152-158. DOI： 10.13422/j.cnki.syfjx.20181092.

HU Kang-li, LI Hua, LIU Wang-hua, et al. Effect of Modified Si Junzitang on Expression of ERK1/2, Akt, Bax in Rats with Cerebral Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 152-158. DOI： 10.13422/j.cnki.syfjx.20181092.

摘要

目的：观察四君子汤加减对脑缺血/再灌注损伤大鼠细胞外调节蛋白激酶1/2（ERK1/2），蛋白激酶B（Akt），细胞淋巴瘤/白血病-2相关X蛋白（Bax）的影响，探讨其保护神经细胞可能作用机制。方法：将55只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组、四君子汤加减低、中、高剂量组（3.69，7.38，14.76 g·kg-1）。采用线栓法制备大脑中动脉阻塞（MCAO）模型，治疗7 d后处死，取大鼠缺血侧脑组织，运用蛋白免疫印迹法（Western blot）检测磷酸化ERK1/2（p-ERK1/2），磷酸化Akt（p-Akt），Bax蛋白含量。应用实时荧光定量聚合酶链式反应法（Real-time PCR）测定脑缺血再灌注后脑组织内ERK1/2，Akt，Bax mRNA的表达变化。结果：与假手术组比较，模型组p-ERK1/2，p-Akt蛋白及mRNA表达显著下调（P<0.01）；Bax蛋白及mRNA表达显著上调（P<0.01）；与模型组比较，四君子汤加减低、中、高剂量组p-ERK1/2，p-Akt蛋白及mRNA表达明显上调，Bax蛋白及mRNA表达明显下调，差异具有统计学意义（P<0.01）。结论：四君子汤加减可能通过上调p-ERK1/2，p-Akt，下调Bax蛋白表达发挥脑保护作用。

Abstract

Objective: To observe the effect of modefied Si Junzitang on the expression of extracellular signal-regulated kinase1/2(ERK1/2)

protein kinase B(Akt) and B cell lymphoma/lewkmia-2 assaciated X protein(Bax) in rats with cerebral ischemia/reperfusion injury

and to explore its possible protective mechanism on nerve cells. Method: Totally 55 male SD rats were randomly divided into sham operation group

model group

edaravone group

modefied Si Junzitang low-dose group

middle-dose group

and high-dose group (3.69

7.38

14.76 g·kg-1). The middle cerebral artery occlusion (MCAO) model was induced by suture method

after 7d treatment

the rats were sacrificed and ischemic side brain tissues were taken. Phosphorylation of ERK1/2 (p-ERK1/2)

p-Akt and p-Bax protein contents were detected by Western blot; the mRNA expression levels of ERK1/2

Akt and Bax were detected by real time fluorescence quantitative polymerase chain reaction(Real-time PCR). Result: As compared with sham operation group

the p-ERK1/2

p-Akt and p-Bax protein and mRNA expression levels were significantly reduced in model group(P<0.01). As compared with the model group

the protein and mRNA expression levels of p-ERK1/2 and p-Akt were up-regulated

the expression of protein and mRNA of Bax were significantly reduced in low-dosed group

middle-dosed group

high-dosed group

with statistically significant difference (P<0.01). Conclusion: Modefied Si Junzitang can protects brain with cerebral ischemia/reperfusion injury by up-regulating the expression of p-ERK1/2 and p-Akt. reducing the expression of Bax.

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