LIU Peng-peng, CHEN Jiang-ning, JIA Tian-zhu. Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 34-38.
LIU Peng-peng, CHEN Jiang-ning, JIA Tian-zhu. Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 34-38. DOI: 10.13422/j.cnki.syfjx.20181105.
Objective: This study aimed to analysis the diversity of endophytic fungal communities in Astragali Radix
and provide a reference for study on the directional processing technology of active ingredients in this herb. Method: The 18S rDNA NS1-Fung region of endophytic fungal communities in Astragali Radix was sequenced by Illumina MiSeq high-throughput sequencing technology
abundance of species and other bioinformation were analyzed. Result: The numbers of effective sequences and operational taxonomic units for samples were 29 677 and 188
respectively.The number of sequencing was close to saturation
and the sequencing data volume was reasonable.The main endophytic fungi of Astragali Radix belonged to 5 genera
such as Hemileia
Gibberella
Ascobolus
Pseudogymnoascus and Aspergillus. Conclusion: By and large
the diversity of endophytic fungi in Astragali Radix is low.Hemileia and Gibberella are the dominant endophytic fungi in Astragali Radix.