Objective: To investigate the therapeutic mechanism of Yinchen Wulingsan based on network pharmacology. Method: Based on the Traditional Chinese Medicine Systems Pharmacology Database

the Protein Databank and the String database

the chemical compositions of Yinchen Wulingsan

corresponding targets and diseases were obtained respectively. Meanwhile

compound-target network

as well as target-disease network and protein-protein interaction network

were constructed. According to biological information annotation dotabase(DAVID) annotation databases

gene ontology biology process (GO-BP) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the targets were performed. Result: Totally 58 components

103 targets

11 hub targets and 262 diseases were obtained. The key targets involved tumor necrosis factor (TNF)

peroxidase synthetase 2 (PTGS2)

and estrogen receptor (ESR1)

etc. The diseases mainly involved tumor

immune and endocrine. There were 146 GO-BP entries

including signal transduction entries

transcription and translation entries

proliferation promotion and apoptosis inhibition entries

cellular component entries

and oxidative stress entries. Besides

there were 108 KEGG pathways

involving tumor

virus

parasite and so on. Conclusion: The targets and mechanism corresponding to compounds of Yinchen Wulingsan were studied systematically in this study

providing ideas for further clinical researches and new drug development.