小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响

兰瑞; 张勇; 马云枝; 吴涛; 郑海忠; 王保琦; 武继涛

doi:10.13422/j.cnki.syfjx.20181137

您当前的位置：

首页 >

文章列表页 >

小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响

药理 | 更新时间：2024-01-04

- 小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响
- Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury
- 中国实验方剂学杂志 2018年24卷第15期页码：149-154
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81503422）
- DOI：10.13422/j.cnki.syfjx.20181137
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
移动端阅览
兰瑞, 张勇, 马云枝, 等. 小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响[J]. 中国实验方剂学杂志, 2018,24(15):149-154.

LAN Rui, Zhang YOng, MA Yun-zhi, et al. Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 149-154.
兰瑞, 张勇, 马云枝, 等. 小续命汤对急性脑缺血再灌注损伤BDNF,GDNF表达的影响[J]. 中国实验方剂学杂志, 2018,24(15):149-154. DOI： 10.13422/j.cnki.syfjx.20181137.

LAN Rui, Zhang YOng, MA Yun-zhi, et al. Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 149-154. DOI： 10.13422/j.cnki.syfjx.20181137.

摘要

目的：观察小续命汤对大鼠急性脑缺血再灌注损伤后脑源性神经营养因子（brain derived neurotrophic factor，BDNF），胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor，GDNF）表达的影响。方法：60只雄性SPF级SD大鼠随机分为假手术组、模型组、小续命汤低、中、高（15，30，60 g·kg-1）剂量组。各组大鼠于造模前3 d开始给药直至观察节点结束，采用大脑中动脉线栓法建立脑缺血再灌注损伤模型，采用苏木素-伊红（HE）染色，Nissl染色观察脑缺血再灌注损伤，采用蛋白免疫印迹法（Western blot）和免疫组织化学法检测脑缺血再灌注损伤后的BDNF，GDNF蛋白表达。结果：与模型组比较，小续命汤高、中剂量组可显著改善其神经功能缺损（P<0.05），减轻脑缺血再灌注引起的缺血性损伤；小续命汤高、中剂量组还可显著上调BDNF，GDNF蛋白表达（P<0.05）。结论：小续命汤可能通过上调脑缺血再灌注后缺血半暗带区BDNF，GDNF的表达发挥脑保护的作用。

Abstract

Objective: To observe the effects of Xiao Xumingtang (XXMT) on brain derived neurotrophic factor (BDNF)and glial cellderived neurotrophic factor (GDNF)expression levels after acute cerebral ischemia reperfusion injury in rats. Method: A total of 60 male Sprague-Dawley rats were randomly divided into 5 groups:sham operation group (Sham)

model group (IR)

and XXMT low dose

middle dose and high dose groups (15

60 g·kg-1·d-1). The Drugs were given from 3 days efore modeling to the end of observation nodes. Cerebral ischemia reperfusion injury models were established by using middle cerebral artery occlusion. Then the pathological changes were estimated by hematoxylin-eosin (HE) staining and Nissl staining at 24 h after reperfusion. BDNF and GDNF expression levels after cerebral ischemia reperfusion injury were quantitatively determined by Western blot and immunohistochemistry. Result: The results indicated that XXMT middle and high dose (30

60 g·kg-1·d-1) treatment significantly improved neurologic impairment (P<0.05)

reduced ischemic injury

and up-regulated the expression levels of BDNF and GDNF as compared with IR group(P<0.05). Conclusion: These findings indicated that XXMT treatment may exert the neuroprotective effect on cerebral ischemia by up-regulating expression levels of BDNF and GDNF.

关键词

Keywords

references

浏览量

291

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于NLRP3/GSDMD/Caspase-1通路探究小续命汤联合电针对脑缺血/再灌注损伤的改善作用

基于UPLC-Q-TOF-MS/MS的小续命汤基准样品化学成分及指纹图谱分析

经典名方小续命汤的历史沿革及临床应用考证

小续命汤联合超早期针刺督脉对于脑缺血再灌注模型大鼠自噬相关蛋白NF-κB p65的影响