Objective: To determine the contents of macroelements and trace elements in bone marrow powder (BMPD)

bone marrow oil (BMO)

bone marrow protein (BMP) and bone protein (BP) from 4 types of Xinjiang characteristic animals:sheep

bovine

horse

and camel

measure the molecular weight of BMP

and provide a reference for improving the quality standard of traditional animal drugs. Method: Inductively coupled plasma-atomic emission spectrometry(ICP-OES) was used to determine the contents of 15 elements including Ca

K

Na

B

P

Ba

Cr

Cu

Fe

Mg

Mn

Ni

Sr

Zn and Si;1-DE electrophoresis was used to identify the molecular weight of BMP. Result: Four types of animals BMP bands were all shown at 66 kDa; the trace elements showed good linearity; detection limits range was 0.13-3.99 μg·L -1

with precision RSD of 0.2%-2.7% and recovery rate of 92.5%-101.4%. A large number of constant and trace elements were enriched in BP and BMP

of which horse BMP showed the highest level of beneficial trace elements

with a content of 24.92

10.83 mg·g-1 respectively for Ca and P;the content of Na

K

Ca

P

Mg was as follows:BP>BMP>BMPD>BMO;the content of Fe

Cu

Zn

Mn and other elements:BMP>BP>BMPD>BMO. Conclusion: The established ICP-OES method was suitable and highly sensitive for the comparative analysis of trace elements in BM and bone samples from different sources of domestic animals. It was suggested that

protein and peptide should be extracted intensively to improve not only the comprehensive utilization of bone and bone marrow

and also could provide theoretical basis for the research and development of functional foods and medicines.