Objective: To explore the influence of Rehmanniae Radix Praeparata decoction processed with different techniques on the ovulation function of female rats

and to provide reference for the processing and clinical use of this decoction pieces. Method: Steamed method (one steaming and one drying) and traditional method (nine steaming and nine drying) were used to prepared Rehmanniae Radix Praeparata

respectively.Female adult rats with a normal estrus cycle were selected as study subjects

blank group

steaming group and traditional group were set up.The effects of two Rehmanniae Radix Praeparata decoctions on the estrous cycle

ovarian tissue morphology and serum hormone levels in female rats were observed.The doses in the steaming group and the traditional group were 3.6 g·kg-1.The HPLC fingerprints of Rehmanniae Radix Praeparata was established

fingerprints and pharmacodynamic indexes were used to investigate effect of Rehmanniae Radix Praeparata on ovulation of female rats. Result: Compared with the blank group

serum estradiol and progesterone levels in the steaming group were significantly decreased(P<0.05)

the estrous cycle was significantly prolonged(P<0.05)

and the number of atresia follicles in the ovary was significantly increased(P<0.05);the change of each index in the traditional group was not obvious.Verbascoside and effect of inhibiting ovulation of Rehmanniae Radix Praeparata processed steamed method in rats positively correlated;peak 3 and 5 in HPLC fingerprints of Rehmanniae Radix Praeparata processed with traditional method were negatively correlated with inhibition of ovulation. Conclusion: Rehmanniae Radix Praeparata processed by steamed method has a certain inhibitory effect on ovulation of normal female rats.Rehmanniae Radix Praeparata processed by traditional method has some advantages in ovulation function.