铁线蕨总黄酮提取物对哮喘模型大鼠血清代谢物的影响

阿依古·; 吐热克; 阿不都热依木·; 玉苏甫; 巴吐尔·; 买买提明; 米合勒阿依·; 艾克帕; 麦合苏木·; 艾克木

doi:10.13422/j.cnki.syfjx.20181305

您当前的位置：

首页 >

文章列表页 >

铁线蕨总黄酮提取物对哮喘模型大鼠血清代谢物的影响

药物代谢 | 更新时间：2024-01-04

- 铁线蕨总黄酮提取物对哮喘模型大鼠血清代谢物的影响
- Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model
- 中国实验方剂学杂志 2018年24卷第14期页码：76-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆维吾尔自治区中医民族医药管理局人才培养计划项目（2016-03）
- DOI：10.13422/j.cnki.syfjx.20181305
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
移动端阅览
阿依古·, 吐热克, 阿不都热依木·, 等. 铁线蕨总黄酮提取物对哮喘模型大鼠血清代谢物的影响[J]. 中国实验方剂学杂志, 2018,24(14):76-83.

AYIGU·, Tureke, ABDIRYIM·, et al. Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 76-83.
阿依古·, 吐热克, 阿不都热依木·, 等. 铁线蕨总黄酮提取物对哮喘模型大鼠血清代谢物的影响[J]. 中国实验方剂学杂志, 2018,24(14):76-83. DOI： 10.13422/j.cnki.syfjx.20181305.

AYIGU·, Tureke, ABDIRYIM·, et al. Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 76-83. DOI： 10.13422/j.cnki.syfjx.20181305.

摘要

目的：探讨铁线蕨总黄酮提取物(TXJF)对哮喘大鼠血清代谢物的影响。方法：在建立哮喘大鼠模型的基础上，哮喘模型大鼠灌胃给予TXJF，观测肺泡灌洗液(BALF)中白细胞总数及淋巴细胞(Ly)，中性粒细胞(Neu)，嗜酸性粒细胞(Eos)的百分比；检测肺组织病理变化；测定大鼠血清中免疫球蛋白E(IgE)，白细胞介素-4(IL-4)，IL-5的浓度。利用核磁共振氢谱技术(1H-NMR)检测，建立大鼠血清代谢物的指纹图谱，运用偏最小二乘法-判别分析(PLS-DA)和正交偏最小二乘法-辨别分析(OPLS-DA)观察各组血清中代谢产物的差异性。结果：与哮喘模型组比较，TXJF给药组BALF中白细胞总数及炎症细胞百分比均明显下降、肺组织病理情况有明显改善。TXJF高、中剂量组血清IgE，IL-4，IL-5浓度较哮喘模型组明显下降。PLS-DA结果显示，哮喘模型组与正常组，TXJF给药组轮廓区分明显分开。与正常组比较，哮喘模型组血清中亮氨酸、糖蛋白、甘氨酸、丙氨酸等代谢产物含量均显著下降；乳酸、极低密度脂蛋白(VLDL)和丙酮等含量显著升高。TXJF高剂量组血清中的亮氨酸、丙氨酸、糖蛋白、甘氨酸等化合物含量较哮喘模型组均出现升高；丙酮、乳酸、不饱和脂肪酸及VLDL等化合物含量减少。结论： TXJF具有改善哮喘大鼠模型肺组织病理学变化，降低血清IgE，IL-4，IL-5浓度且能影响血清代谢物的作用；作用机制可能与其参与哮喘模型大鼠体内的氨基酸代谢、能量代谢、脂肪代谢和糖代谢等途径有关。

Abstract

Objective: To investigate the effect of total flavonoids extract of Adiantum capillus-veneris (TXJF) on serum metabolites of asthmatic rats. Method: Based on the establishment of asthmatic rat model

asthma model rats were given TXJF by intragastric administration

then to observe the percentage of leukocyte in bronchoalveolar lavage fluid(BALF) and pathological changes of lung tissue;levels of immunoglobulin E(IgE)

interleukin-4 (IL-4) and IL-5 in serum of rats were detected.1H-NMR was employed to establish fingerprints of serum metabolites in rats

partial least squares discriminant analysis(PLS-DA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were adopted to analyze the differences in serum metabolites. Result: Compared with asthma model group

total number of leukocytes and inflammatory cells in BALF of TXJF administration group were decreased and the pathological changes of lung tissue were significantly alleviated.The levels of IgE

IL-4 and IL-5 in serum from TXJF high and middle dose groups were significantly lower than those in the asthma model group.The results of PLS-DA showed that the contour differentiation between the asthma model group and the normal group

the TXJF administration group was distinctly separated.Compared with the normal group

the levels of leucine

glycoprotein

glycine

alanine and other metabolites in serum of the asthma model group were decreased;the contents of lactic acid

very low density lipoprotein(VLDL) and acetone were increased(P<0.05).In the TXJF high dose group

the contents of leucine

alanine

glycoprotein

glycine and other compounds were all higher than those of the asthma model group;the contents of acetone

lactic acid

unsaturated lipids

VLDL and other compounds were decreased(P<0.05). Conclusion: TXJF can improve the pathological changes of lung tissue in asthmatic rats

decrease concentrations of IgE

IL-4 and IL-5 in serum and affect the effect of serum metabolites;its mechanism may be related to amino acids metabolism

energy metabolism

fat metabolism and glucose metabolism in asthmatic rat model.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于传统品质评价的淡豆豉质量分析

基于¹H-NMR代谢组学技术考察五味子鲜果采后“杀青”的代谢轮廓变化

基于“方证对应”理论探讨主动脉弓缩窄致心力衰竭大鼠模型的中医证型与代谢标志物

瓜拉纳化学成分的UPLC-Q-TOF-MS分析

干寒环境对大鼠尿液与血浆代谢成分的影响