Objective: To establish a analytical method combining graphene-stir bar sorptive extraction (SBSE) and chromatography-mass spectrometry (GC-MS) for the active components of flavonoids in Camellia chrysantha flos and the relative content of total flavonoids.Method: Activated carbon from rice straw was made to prepare the graphene polymer

and the factors affecting SBSE (such as selection of extraction coatings

extraction temperature and time) were optimized. Then GC-MS was used to identify the active components of flavonoids in C. chrysantha flos and determine the relative content of total flavonoids by using peak area normalization method under the optimized conditions.Result: Flavonoids in C. chrysantha flos were identified as catechin

epigallocatechin (EGC)

catechol (C)

quercetin-7-O-glucoside

rutin

isoquercitrin

quercetin

kaempferol

anthocyanins

epigallocatechin gallate (CG)

and epigallocatechin gallate (CG) etc. The relative content of total flavonoids (mainly including quercetin and kaempferol) reached 3.0%. After 6 tests of the same sample

the total peak area of the total flavonoids in C. chrysantha flos was used as the evaluation index

with a relative standard deviation (RSD) of 1.0%.Conclusion: The method separated and identified 24 components by SBSE-GC-MS

and the results indicated good reproducibility and appropriateness of the method to test the flavonoids in C. chrysantha flos

providing theoretical reference for further studies on flavonoids in C. chrysantha flos.