Objective: To investigate the anti-tumor effect of quercetin self-nanoemulsifying drug delivery system (Q-SNEDDS) on mice sarcomarosarcoma cell S180 and explore its molecular mechanism. Method: Q-SNEDDS and Blank-SNEDDS were prepared

and then their particle size and Zeta potential were measured respectively. The in vivo experiment was divided into 6 groups:blank group

cyclophosphamide(CTX) group (20 mg·kg-1)

high dose Q-SNEDDS group (50 mg·kg-1)

middle dose Q-SNEDDS group (25 mg·kg-1)

low dose Q-SNEDDS group (12.5 mg·kg-1) and quercetin control group (50 mg·kg-1). S180 bearing mice models were established by using tumor cell transplantation method. After 10 days of drug administration

the inhibition rate and indexes of organ (liver

spleen and thymus) were measured in all groups. B-cell lymphoma-2 (Bcl-2)

Bcl-2 associated X protein (Bax)

and protein kinase B (Akt) mRNA expression levels of S180 were measured by Real-time quantitative polymerase chain reaction (Real-time PCR). The protein expression levels of Bax

Bcl-2

Akt and p-Akt were measured by Western blot method. Result: Q-SNEDDS

CTX and quercetin groups could inhibit the growth of tumor cells as compared with blank group

and the inhibition rate in Q-SNEDDS groups was higher than that in Quercetin group (P<0.05). The organ indexes of CTX group were lower than those in blank group (P<0.05) and the indexes of Q-SNEDDS group were higher than those in CTX group (P<0.05). As compared with blank group

Q-SNEDDS up-regulated Bax mRNA and protein expression (P<0.05). Q-SNEDDS down-regulated Bcl-2 mRNA and protein expression (P<0.05)

down-regulated Akt mRNA and p-Akt protein

but Akt protein expression didn't change obviously. Conclusion: Q-SNEDDS played the anti-tumor role in vivo

and the effect was superior to quercetin. Its mechanism may be associated with inhibiting Akt signaling pathway.