Objective: To observe the effect of compound phyllanthus urinsria Ⅱ (CPU Ⅱ) on the proliferation of hepatocellular carcinoma Huh7 cells and the transcription of insulin-like growth factor-1 receptor (IGF-1R) signaling pathway

and explore the mechanism of action of CPU Ⅱ in inhibiting liver cancer. Method: Small interfering RNA (siRNA) was transfected into Huh7 cells to construct hepatocellular carcinoma Huh7 cells that inhibited insulin-like growth factor-1 receptor (anti-IGF-1R Huh7). IGF-1R Huh7 cells with stable expression

namely Huh7 cells of hepatocellular carcinoma and Huh7 cells of hepatocellular carcinoma that inhibited insulin-like growth factor-1 receptor

were divided into four groups:control group

high and low-dose CPU Ⅱ groups (3.0

1.5 g·L-1) and 5-fluorouracil(5-FU) (0.03 g·L-1). The effect of drugs on the proliferation of each group were determined by methylthiazolyl tetrazolium(MTT)assay. Real-time PCR was used to detect the mRNA expression of IGF-1R and its downstream genes. Result: The mRNA expression of Huh7 IGF-1R and the lowest expression level of Huh7 were detected by Real-time PCR and Western blot. The cells were selected for experiment. The results of MTT showed that CPU Ⅱ could inhibit the proliferation of Huh7 cells and Huh7 cells that inhibit IGF-1R; after siRNA inhibited IGF-1R gene expression

CPU Ⅱ could continue the proliferation of Huh 7 cells; and the mRNA expressions of IGF-1R

Phosphatidylinositol 3-kinase (PI3K)and Serine-threonine protein kinase 3(Akt)further decreased compared with the control group (P<0.05). Conclusion: CPU Ⅱ can inhibit the proliferation of Huh7 cells. The mechanism may be related to the inhibition of IGF-1R and its downstream mRNA transcription.