Objective: To investigate intestinal absorption properties of active ingredients in total flavonoids extract of Hosta plantaginea dried flowers and its composite particles. Method: Everted gut sac model was employed.The duodenum

jejunum

ileum and colon of rats were selected as intestinal segment for research.The apparent permeability coefficient(Papp) was used to evaluate the intestinal absorption characteristics of active ingredients from total flavonoids extract of H. plantaginea dried flowers and its composite particles in different intestinal segments and different concentrations. Result: Under different drug concentrations

kaempferol-3-O-sophoroside and kaempferol-3-O-rutinoside were absorbed in all intestinal segments;at the same concentration

their absorption in the jejunum were the best. Conclusion: The composite particles of total flavonoids extract of H. plantaginea dried flowers changes the intestinal absorption characteristics of kaempferol-3-O-sophoroside and kaempferol-3-O-rutinoside

and it can notably enhance their absorption in the colon.The composite particles can significantly increase the absorption of total flavonoids extract of H. plantaginea dried flowers in the colon.