Objective: To establish an LC-MS/MS method for simultaneous determination of the four effective components(paederosidic acid

paederoside

paederosidic acid methyl ester

and asperuloside) in Paederia scandens extracts. Method: A Venusil MP C18 column (2.10 mm×50 mm

5 μm)was used

with methanol(A)-water(B) as the mobile phase for gradient elution(0-0.3 min

10% A;0.3-0.8 min

10%-95%A;0.8-3.0 min

95%A;3.0-3.2 min

95%-10%A;3.2-4.5 min

10% A). The flow rate was 0.35 mL·min-1

the column temperature was room temperature

and the injection volume was 10 μL. Electrospray ionization(ESI) source was used. Quantization was performed using multiple reaction monitoring(MRM) mode and positive ionization condition. Mass spectrometric parameters of spectrometer and 4 effective components were optimized for testing and quantitative analysis. Result: Based on the instrument precision

linear range

repeatability

stability

recovery and other methodological validations

the results were in conformity with relevant standards of quantitative analysis. The calibration curves of paederosidic acid

paederosidic

methyl paederosidate and asperuloside showed a good linearity (r ≥ 0.994 7).The average recoveries of these four components were 90.8%-112.1%

with RSDs (n=6) of no more than 5.1%. Conclusion: The method is simple

rapid

accurate and reliable

and suitable for the quantitative analysis of four effective components in the extract of P. scandens.