Objective: To develop an HPLC-CAD method for simultaneous determination of six ingredients in Gushukang capsule

namey naringin

epimedin B

epimedin C

icariin

5-hydroxymethylfurfural(5-HMF) and tanshinoneⅡA

in order to provide references for its quality control. Method: By HPLC-CAD

chromatographic separation was performed on a Agilent ZORBAX Eclipse XDB C18 column(4.6 mm×250 mm

5 μm)

with acetonitrile (A)-0.05% formic acid (B) as the mobile phase for gradient elution(0-10 min

10%A;10-25 min

10%-25%A;25-30 min

25%-30%A;30-45 min

30%-60%A;45-50 min

60%-75%A)

the flow rate was 0.8 mL·min-1

the nebulization temperature of detector was 35℃

the column temperature was maintained at 35℃

and the injection volume was 10 μL

so as to determine the 6 contents of Gushukang capsule. Result: Naringin

epimedin B

epimedin C

icariin

5-HMF

and tanshinoneⅡA were linear within the ranges of 0.006-0.120 μg(r=0.999 7)

0.041-0.820 μg(r=0.999 9)

0.011-0.220 μg(r=0.999 6)

0.022-0.440 μg(r=0.999 8)

0.012-0.240 μg(r=0.999 8) and 0.005-0.100 μg(r=0.999 8)

respectively. The average recoveries were 99.26%

97.99%

98.17%

99.34%

98.26% and 99.25%

and the RSDs were 1.7%

1.0%

1.6%

1.4%

1.9% and 1.3%

respectively. Conclusion: The method is accurate

convenient

sensitive and reproducible for quality control of multicomponent in Gushukang capsule.