Objective: To establish a co-infections cell model of Guizhitang syndrome base on the principle of the correspondence between prescription and syndrome

in order to accumulate more data on animal model of Guizhitang syndrome. Method: The effect of Guizhitang drug serum on the expression of Toll-like receptors(TLRs) and downstream signaling components in NR8383 was studied. NR8383 cells was stimulated with 25

50

100 mg·L-1 of Poly (I:C) and 1

5

10 mg·L-1 lipopolysaccharides(LPS)

respectively. The supernatant of the former was collected after 3

6

12

24 h

and that of the later was collected after 6

12

24

48 h

so as to measure the inflammatory factors

such as tumor necrosis factor (TNF)-α or interleukin(IL)-1β. After suitable dose and treatment time were selected

five ratios of Poly(I:C)-LPS were used to provoke NR8383 cells with or without Guizhitang containing serum. The expressions of TLR2

TLR3

TLR4 and TLR6 mRNA in NR8383 cells

TNF-α and IL-1β in culture supernatant were compared. The experimental results were evaluated based on the above six indexes

and the most obvious stimulation effect and intervention effect of Poly (I:C)-LPS ratio were selected. Result: The selected stimulation time was 6 h

when Poly (I:C) was below 50 mg·L-1

and LPS at 1 mg·L-1

which was the appropriate stimulus concentration. Among 10:1

20:1

30:1

40:1 50:1 ratios of Poly(I:C)-LPS

the simulation of 50:1 group was more remarkable than other groups(P<0.01). Furthermore

the intervention of Guizhitang in 10:1 group was more significant than other groups(P<0.01). Conclusion: The results suggest that NR8383 cells stimulated with Poly(I:C)-LPS (10:1) can be used as a syndrome cell model of Guizhitang for further study of Guizhitang and similar prescriptions.