二陈汤加味对慢性阻塞性肺疾病细支气管壁细胞外基质重塑的影响

吴珂; 尚立芝; 谢文英; 张静; 王俊月; 包永生; 季书

doi:10.13422/j.cnki.syfjx.20181425

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二陈汤加味对慢性阻塞性肺疾病细支气管壁细胞外基质重塑的影响

药理 | 更新时间：2024-01-04

- 二陈汤加味对慢性阻塞性肺疾病细支气管壁细胞外基质重塑的影响
- Effect of Modified Erchentang on Extracellular Matrix Remodeling in Bronchiole of Rats with Chronic Obstructive Pulmonary Disease
- 中国实验方剂学杂志 2018年24卷第14期页码：122-127
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目（81573881）;郑州市科技领军人才项目（121PLJRC535）;河南省高等学校重点科研项目（15A360030）
- DOI：10.13422/j.cnki.syfjx.20181425
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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吴珂, 尚立芝, 谢文英, 等. 二陈汤加味对慢性阻塞性肺疾病细支气管壁细胞外基质重塑的影响[J]. 中国实验方剂学杂志, 2018,24(14):122-127.

WU Ke, SHANG Li-zhi, XIE Wen-ying, et al. Effect of Modified Erchentang on Extracellular Matrix Remodeling in Bronchiole of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 122-127.
吴珂, 尚立芝, 谢文英, 等. 二陈汤加味对慢性阻塞性肺疾病细支气管壁细胞外基质重塑的影响[J]. 中国实验方剂学杂志, 2018,24(14):122-127. DOI： 10.13422/j.cnki.syfjx.20181425.

WU Ke, SHANG Li-zhi, XIE Wen-ying, et al. Effect of Modified Erchentang on Extracellular Matrix Remodeling in Bronchiole of Rats with Chronic Obstructive Pulmonary Disease[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 122-127. DOI： 10.13422/j.cnki.syfjx.20181425.

摘要

目的：观察二陈汤加味对慢性阻塞性肺疾病(COPD)模型大鼠细支气管结构重塑的作用及其机制。方法： 50只SD大鼠随机分为正常组、模型组、二陈汤加味低、中、高剂量组(5，10，20 g·kg-1)，共5组，每组10只。以香烟烟熏加内毒素脂多糖(LPS)制备大鼠COPD模型。造模成功后，各观察组灌胃给药，检测肺功能，实时荧光定量聚合酶链式反应(Real-time PCR)检测肺组织中基质金属蛋白酶-1(matrix metalloproteinases，MMP-1)，MMP-9及金属蛋白酶组织抑制剂-1(tissueinhibitor of metalloproteinase，TIMP-1) mRNA表达，免疫组织化学法(immunohistochemistry，IHC)检测MMP-1，MMP-9，TIMP-1以及Ⅰ型和Ⅲ型胶原在细支气管中的表达。结果：与正常组比较，模型组肺组织匀浆MMP-1，MMP-9，TIMP-1 mRNA表达均显著增强(P<0.01)，细支气管壁MMP-1，MMP-9，TIMP-1，Ⅰ型及Ⅲ型胶原蛋白的表达均显著增强(P<0.05)。与模型组比较，中、高剂量组肺组织匀浆MMP-1，MMP-9，TIMP-1 mRNA表达显著减弱(P<0.01)，细支气管壁中、高剂量组Ⅰ型和Ⅲ型胶原蛋白的表达明显减弱(P<0.05)。结论：二陈汤加味对细支气管管壁细胞外基质(extracellular matrix，ECM)中的胶原有抑制作用。其机制可能与抑制MMP-1，MMP-9，并协调性抑制TIMP-1的表达，阻止细支气管ECM胶原的沉积有关。

Abstract

Objective: To study the effect of modified Erchentang on extracellular matrix remodeling in the bronchiole of rats with chronic obstructive pulmonary disease. Method: Fifty SD rats were randomly divided into normal group

model group

and modified Erchentang groups (5

20 g·kg-1)

with 10 in each group. The COPD model of rats was prepared with cigarette smoke and lipopolysaccharide (LPS). After successful modeling

each observation group was given drugs by gavage (ig) and tested for lung function. The expressions of matrix metalloproteinase-1 (MMP-1)

MMP-9 and tissue inhibitor metalloproteinase-1 (TIMP-1) mRNA were detected by quantitative Real time PCR (Real-time PCR) assay. The expressions of MMP-1

MMP-9

TIMP-1

Type Ⅰ and Type Ⅲ collagen protein were detected by immunohistochemistry (IHC) techniques. Result: The expressions of MMP-1

MMP-9 and TIMP-1 mRNA were increased significantly (P<0.01)

the expressions of MMP-1

MMP-9

TIMP-1

Type Ⅰ and Type Ⅲ collagen in bronchiole tissue were higher significantly (P<0.05) in model group than those in normal group. Compared with model group

the expressions of MMP-1

MMP-9

TIMP-1 mRNA were decreased significantly (P<0.01)

while the protein expressions of MMP-1

MMP-9

TIMP-1

Type Ⅰ and Type Ⅲ collagen were decreased significantly (P<0.05) in middle and high-dose modified Erchentang groups. Conclusion: Modified Erchentang has an inhibitory effect on collagen in the extracellular matrix(ECM) of bronchioles. Its mechanism may be related to suppressing the expressions of MMP-1

MMP-9

coordinately inhibiting the expression of TIMP-1 and preventing bronchiolar ECM collagen deposition.

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