Objective:Based on the HPLC specific chromatograms and the contents of main chemical components

to discuss the change law of substance basis in Paeoniae Radix Alba decoction pieces before and after being processed. Method:RP-HPLC was employed with ZORBAX SB-C18 column(4.6 mm×250 mm

5 μm)

a gradient mobile phase of acetonitrile-0.05% phosphoric acid aqueous solution was eluted

the column temperature was 30℃ and the flow rate was 1.0 mL·min-1

detection wavelength was set at 254 nm

the method for characterizing the characteristics of Paeoniae Radix Alba decoction pieces was established;at the same time

gallic acid and other 5 ingredients were simultaneously determined at 230 nm.The contents of 1

2

3

4

6-pentagalloylglucose and benzoic acid were simultaneously determined with mobile phase of acetonitrile-0.05% phosphoric acid aqueous solution(18:82). Result:Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces were marked with twelve characteristic peaks

and eight of them were assigned.After being processed

the contents of paeoniflorin and albiflorin decreased by 6% and 7%

respectively;the content of tannins increased significantly

the contents of gallic acid

1

2

3

4

6-pentagalloylglucose and benzoic acid increased by 28%

26% and 53%

respectively;while the contents of other components did not change significantly. Conclusion:According to the specific chromatograms and contents of main chemical components in this article

it can provide reference for formulation of quality evaluation criteria of Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces

which lays the foundation for further improving the quality evaluation system of them.