Objective: To investigate the chemical constituents of the underground parts of Liriope spicata var. prolifera

further enrich its chemical information and provide theoretical guidance for its development and utilization. Method: The underground parts of L. spicata var. prolifera were extracted by 60% ethanol. The chemical constituents in 60% ethanol extract were then isolated and purified by macroporous adsorption resin

positive and reverse silica gel

MCI gel and sephadex gel. Their structures were determined by detailed analysis of their physico-chemical properties and spectral data. Result: Twelve compounds were isolated and identified from the underground parts of L. spicata var. prolifera

including 4 flavonoids(compounds 1-4)

4 phenols and benzoic acid derivatives (compounds 5-8)

2 amide alkaloids (compounds 9

10) and 2 other kinds (compounds 11

12). Their structures were identified as 2'

4

4'-trihydroxychalcone(1)

luteolin(2)

quercitroside(3)

3

5-dihydroxy-7-methoxy-6-methyl-3-(4-hydroxy benzyl) chroman-4-one(4)

2

6-dimethoxy-4-nitrophenol(5)

allylpyrocatechol(6)

3

4-dihydroxyallylbenzene 4-O-[α-L-rhamnopyranosyl(1→6)]-β-D-glucopyranoside(7)

syringic acid(8)

E- feruloyltyramine(9)

tran-p-hydroxy cinnamic acid(10)

(1 S

4 S

5 R

6 R

7 S

10 S)-1

4

6-trihydroxyeudesmane-6-O-β-D-glucopyranoside(11)

and 5-hdroxymethyl-2-furaldehyde (12)

respectively. Conclusion: Compounds 1-3

5-8 and 10-12 were isolated from this genus for the first time. Compounds 4 and 9 were isolated from this plant for the first time.