Objective:To optimize processing technology of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus and establish its HPLC fingerprint. Method:The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus was investigated by model rats with splenasthenic diarrhea

and then the best ratio of Atractylodis Macrocephalae Rhizoma and Aurantii Fructus Immaturus was screened.Taking overall desirability of contents of synephrine and atractylenolide Ⅲ as index

orthogonal test was employed to optimize processing technology

and HPLC fingerprint of this processed product was established. Result:The best ratio of Atractylodis Macrocephalae Rhizoma to Aurantii Fructus Immaturus was 9:1.The factors influenced contents of synephrine and atractylenolide Ⅲ were followed by baking temperature > frying temperature > frying time

optimum processing technology of this processed product was as follows:added Aurantii Fructus Immaturus juice 50 mL(crude drug concentration of 0.2 g·mL-1) into 100 g of Atractylodis Macrocephalae Rhizoma pieces

soaked 24 hours

fried 6 min at 110℃

dried at 40℃ in a oven.Five common peaks were selected in Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus.Among the obtained fingerprint

all of the detected peaks were separated effectively.The accuracy

repeatability and stability of this method were satisfied. Conclusion:The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus can reflect the processing characteristics of increasing efficiency and correcting bias.The established HPLC fingerprint can provide reference for the quality control of this processed product.