Objective:To establish the methods of HPLC fingerprint and quantitative determination of multi-components of bamboo leaves

the synthetical evaluation for six extraction technologies of bamboo leaves were developed by principal component analysis and hierarchical cluster analysis. Method:The optimal conditions of separation and detection were achieved on Diamonsil Plus C18 column(4.6 mm×250 mm

5 μm) with linear gradient elution of acetonitrile-0.025% formic acid water solution at a flow rate of 1 mL·min-1.The detection wavelength was 350 nm

the column temperature was 30℃.Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition) and SPSS 22.0 were used to analyze the data. Result:The content ranges of 4 components(chlorogenic acid

isoorientin

orientin

isovitexin) in six bamboo leaves extract were 0.08-0.30

0.24-1.64

0.26-0.79

0.16-0.79 g·L-1

respectively.A total of 11 common peaks were identified in the HPLC fingerprint and the similarities of the six extract were all >0.89.Six extraction technologies of bamboo leaves were divided into 3 categories according to hierarchical cluster analysis.Two principal components were extracted

whose cumulative contribution rate was 98.9%. Conclusion:The contents of chlorogenic acid

isoorientin

orientin and isovitexin in six bamboo leaves extract have a great difference.Hierarchical cluster analysis and principal component analysis complement and support each other

combining with the similarity evaluation

which can be applied to the optimization of extraction technology of bamboo leaves.Meanwhile

the research can provide reference for industrial development of bamboo leaves.