Objective:To evaluate the quantity of 11 batches of Erzhiwan samples by establishing a HPLC method for simultaneous determination of five characteristic components(specnuezhenide

salidroside

tyrosol

luteolin and wedelolactone) in this preparation. Method:Phenomenex Gemini C18 column(4.6 mm×250 mm

5 μm) was employed with mobile phase of acetonitrile-0.2% formic acid solution for gradient elution(0-15 min

10%-30%A;15-25 min

30%-33%A;25-30 min

33%A)

flow rate was 1 mL·min-1

detection wavelengths were 225 nm and 349 nm

column temperature was set at 30℃. Result:Salidroside

tyrosol

specnuezhenide

luteolin and wedelolactone were well separated with linear ranges of 202.55-12 153

84.55-5 073

244.30-14 657.72

2.56-153.72

2.32-139.2 ng

respectively;their average recovery rates were between 95.61% to 103.47% with RSD<2.0%. Conclusion:This determination of five components is simple

accurate and reproducible for the quality control of Erzhiwan.The contents of these five components in 11 batches of samples are quite different.The content of specnuezhenide in samples is ranged from 0.15 mg·g-1 to 10.22 mg·g-1

which may be caused by the materials

the operative norms and other factors.