Objective: To study the protective effect of bacuchiol on hepatic stellate cells induced by transform growth factor-β(TGF-β) and its mechanism. Method: The activity of each group of cells was detected by methye thiazolye telrazlium(MTT)

in order to screen out the safe and effective concentration of bacuchiol. This experiment was divided into blank group

model group (10 μg·L-1 TGF-β)

bacuchiol group (1×10-5 mol·L-1)

estradiol group (1×10-6 mol·L-1)

and their activities were detected. Superoxide dismutase (SOD)

Glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) of each group were detected by enzyme linked immunosorbent assay(ELISA); Real-time quantitative-PCR (Real-time PCR) was used to detect the changes in matrix metalloproteinase-1 (MMP-1) and collagen-Ⅰ (Col-Ⅰ) mRNA expressions in hepatic stellate cells induced by TGF-β; Western blot method was used to detect the changes of MMP-1 and Col-Ⅰ protein content in hepatic stellate cells induced by TGF-β. Result: The 1×10-5mol·L-1 bacuchiol can effectively improve the activity of SOD and GSH-Px in hepatic stellate cells

and also reduce MDA content in cells and the expressions of Col-Ⅰ and MMP-1 mRNA and protein (P<0.01). Conclusion: Bacuchiol has a protective effect on hepatic stellate cells induced by TGF-β mainly by improving the activity of antioxidant enzymes in cells and reducing the content of MMP-1

Col-Ⅰ mRNA and protein expressions in cells.