Objective:To explore the effect of Erzhiwan on the biological basis of mice of ovariectomized-induced Alzheimer's disease(AD) by proteomic method. Method:Ovariectomized mice were randomly divided into model group

positive control group

high-dose Erzhiwan group and low-dose Erzhiwan group

with 14 mice in each group. Another 14 mice were included in the sham operation group. Six weeks later after the operation

the mice in the sham operation group and the model group were given the same volume of distilled water

high and low-dose Erzhiwan groups were given the corresponding drugs by gavage

with the drug concentration of 2

1 g·kg-1

and the positive control group was injected with estradiol benzoate 0.02 mg every other day. The drug administration lasted for 32 days. Proteins were extracted from the mice's hippocampus

and detected by Nanol-ESI liquid-mass spectrometry system

and then identified by Protein Discovery software; and a quantitative analysis was made for the hippocampal proteins by SIEVE software. PANTHER Classification System was used for gene ontology(GO) analysis of differential proteins

and Kyoto Encyclopedia of Genes and Genomes(KEGG) was used to enrich signal pathways. Result:Compared with the model group

there were more than 80 differentially expressed proteins in the normal group and the high and low-dose Erzhiwan groups. Through the GO analysis

these proteins could be divided into tubulin

heat shock protein

energy metabolism-related protein

brain protection-related proteins and other AD-related proteins. KEGG analysis found that the above differential proteins involved 20 signal pathways. These differential proteins may be the target for Erzhiwan in preventing and treating AD. Conclusion:Erzhiwan can promote the energy metabolism and vesicle transport

while reducing the production of Aβ and the oxidative damage of neurons

so as to achieve the therapeutic effect of AD.