Objective: To Compare the content of catalpol

acteoside

rehmaionoside A

rehmaionoside D

leonuride and polysaccharide between radial striations and non-radial striations of Rehmannia glutinosa during growth with six germplasms(Qinhuai

85-5

Beijing-1

QH-1

1706

Baixuan)

and analyze the component differences and quality characteristics of radial striations in different germplasm resources of R. glutinosa

in order to provides a reference for quality evaluation and geoherbalism of R. glutinosa.Method: The method to determine the contents of catalpol and acteoside consulted Chinese Pharmacopoeia 2015 edition. The contents of rehmaionoside A

rehmaionoside D and leonuride were determined by HPLC. Chromatographic column was Dikma Diamonsil C18(4.6 mm×250 mm

5 μm)

with mobile phase of acetonitrile-water(4:96);flow rate was 1.0 mL·min-1;detection wavelength was 205 nm;column temperature was 30℃;sample size was 20 μL. The content of polysaccharide was determined by phenol sulfate method.Result: There were significant differences in accumulation amount between radial striations and non-radial striations

particularly in tuberous root with different germplasms R. glutinosa. The content of catalpol in radial striations was far higher than that in non-radial striations of 85-5 and 1706

whereas that in non-radial striations was far higher than that in radial striations of Beijing-1 and QH-1.The content of acteoside in radial striations was far higher than that in un-radial striations of 85-5

1706 and QH-1

but with a relatively uniform distribution of the other three germplasms. The content of rehmaionoside A in non-radial striations was higher than that in radial striations evidently of Qinhuai and Beijing-1.The content of rehmaionoside D in non-radial striations was higher than that in radial striations

but it was substantial consistent with the other three germplasms. The content of leonuride was substantial consistent with radial striations and non-radial striations of 85-5

Baixuan

1706;but the content in radial striations was far higher than non-radial striations of Qinhuai

Beijing-1

QH-1

with up to 2-4 times of differences. The content of polysaccharide in non-radial striations was higher than that in radial striations of all six germplasms.Conclusion: The accumulation and distribute of secondary metabolite between radial striations and non-radial striations in the tuberous root of R. glutinosa were decided by germplasms resource to a certain degree.