定心方通过上调CD4CD25Foxp3调节性T细胞表达对PM2.5所致动脉粥样硬化的影响

万强; 刘中勇

doi:10.13422/j.cnki.syfjx.20181732

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定心方通过上调CD4CD25Foxp3调节性T细胞表达对PM2.5所致动脉粥样硬化的影响

药理 | 更新时间：2024-01-04

- 定心方通过上调CD4CD25Foxp3调节性T细胞表达对PM2.5所致动脉粥样硬化的影响
- Effect of Dingxin Recipe in Inhibiting Atherosclerosis Induced by PM2.5 by Up-regulating CD4 CD25 Foxp3 Regulatory T Cells Expression
- 中国实验方剂学杂志 2018年24卷第17期页码：139-144
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81660770）;江西省自然科学基金项目（20161BAB215256）;江西省卫生计生委科技计划项目（20171105）;江西省卫生计生委中医药科研课题项目（2016A083）
- DOI：10.13422/j.cnki.syfjx.20181732
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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万强, 刘中勇. 定心方通过上调CD4CD25Foxp3调节性T细胞表达对PM2.5所致动脉粥样硬化的影响[J]. 中国实验方剂学杂志, 2018,24(17):139-144.

WAN Qiang, LIU Zhong-yong. Effect of Dingxin Recipe in Inhibiting Atherosclerosis Induced by PM2.5 by Up-regulating CD4 CD25 Foxp3 Regulatory T Cells Expression[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 139-144.
万强, 刘中勇. 定心方通过上调CD4CD25Foxp3调节性T细胞表达对PM2.5所致动脉粥样硬化的影响[J]. 中国实验方剂学杂志, 2018,24(17):139-144. DOI： 10.13422/j.cnki.syfjx.20181732.

WAN Qiang, LIU Zhong-yong. Effect of Dingxin Recipe in Inhibiting Atherosclerosis Induced by PM2.5 by Up-regulating CD4 CD25 Foxp3 Regulatory T Cells Expression[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 139-144. DOI： 10.13422/j.cnki.syfjx.20181732.

摘要

目的：观察定心方（DXR）在小鼠吸入PM2.5所致动脉粥样硬化（AS）过程中对CD4+CD25+Foxp3+调节性T细胞（CD4+CD25+Foxp3+Tregs）表达调控的影响。方法：50只载脂蛋白E基因敲除（ApoE-/-）小鼠喂食高脂饲料并吸入严重污染的自然空气，随机分为5组，DXR低、中、高剂量组分别灌胃DXR 9.29，18.59，37.18 g·kg-1·d-1；辛伐他汀组灌胃辛伐他汀5 mg·kg-1·d-1；模型组灌胃等剂量生理盐水；10只C57BL/6J小鼠喂食普通饲料吸入过滤空气设为正常组。16周后检测小鼠血清脂质、酶联免疫吸附测定测定（ELISA）检测白细胞介素-6（IL-6），肿瘤坏死因子-α（TNF-α），白细胞介素-10（IL-10）及转化生长因子-β（TGF-β）水平；流式细胞法检测脾脏CD4+CD25+Foxp3+Tregs数量；苏木素-伊红（HE）染色观察主动脉AS斑块形态学变化；蛋白免疫印迹法（Western blot）检测主动脉AS斑块Foxp3蛋白表达。结果：与正常组比较，模型组小鼠主动脉AS斑块形成，血清脂质，IL-6及TNF-α水平明显升高，IL-10及TGF-β水平明显降低，CD4+CD25+Foxp3+Tregs数量明显减少，Foxp3蛋白表达明显降低（P<0.05）；与模型组比较，辛伐他汀组，DXR中、高剂量组小鼠AS斑块面积明显减小，血清脂质，IL-6及TNF-α水平明显降低，IL-10及TGF-β水平明显升高，CD4+CD25+Foxp3+Tregs数量明显增加，Foxp3蛋白表达明显增加（P<0.05）。结论：DXR可通过上调CD4+CD25+Foxp3+Tregs表达发挥抗PM2.5所致AS功效。

Abstract

Objective:To investigate the effect of Dingxin recipe (DXR) on the expression of CD4+CD25+Foxp3+ regulatory T cells (CD4+CD25+Foxp3+ Tregs) in mice during atherogenesis induced by PM2.5. Method:Fifty western-diet apolipoprotein E knockout (ApoE-/-) mice were exposed to PM2.5 and randomly divided into five groups:model group

low-dose DXR group (9.29 mg·kg-1·d-1)

medium-dose DXR group (18.59 mg·kg-1·d-1)

high-dose DXR group (37.18 mg·kg-1·d-1) and simvastatin group (5 mg·kg-1·d-1). Ten normal-diet C57BL/6J mice were exposed to filtered air and included into control group. Serum levels of lipid

interleukin-6(IL-6)

tumor necrosis factor-α(TNF-α)

IL-10 and transforming growth feactor-β(TGF-β) were detected by enzyme linked immunosorbent assay(ELISA)

CD4+CD25+Foxp3+ Tregs number in the spleen

atherosclerotic lesion was detected by htoxylin eosin(HE) and Foxp3 protein expression was detected by Western blot in aorta were measured after 16 weeks. Result:Compared with the control group

atherosclerotic lesion

serum levels of lipid

IL-6 and TNF-α were significantly increased

while serum levels of IL-10 and TGF-β

CD4+CD25+Foxp3+ Tregs number and Foxp3 protein expression were significantly decreased in the model group (P<0.05). Compared with the model group

atherosclerotic lesion

serum levels of lipid

IL-6 and TNF-α were significantly decreased

while serum levels of IL-10 and TGF-β

CD4+CD25+Foxp3+ Tregs number and Foxp3 protein expression were significantly increased in the simvastatin group

medium-dose DXR group and high-dose DXR group(P<0.05). Conclusion:DXR inhibits atherosclerosis induced by PM2.5 by up-regulating CD4+CD25+Foxp3+ Tregs expression.

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