Objective:To research the effect of mangiferin on serum uric acid and intestinal urate transporter ATP-binding cassette superfamily G (White) member 2(ABCG2) gene and protein expressions in the hyperuricemic mice induced by urate. Method:A total of 60 Kunming mice were randomly divided into six groups

namely the normal group

the model group

the mangiferin (1.5

3.0

6.0 mg·kg-1) groups and the positive drug benzbromarone (25.0 mg·kg-1) group

with 10 mice in each group. Except for the normal group

the hyperuricemia model was induced by intraperitoneal injection with urate at a dosage of 150 mg·kg-1. Meanwhile

mangiferin and benzbromarone were administered intragastrically. Two weeks later

the phosphotungstic acid method was used to detect the uric acid of mice; reverse transcription PCR and Western blot were performed to detect jejunal and ileac ABCG2 mRNA and protein expressions of mice; and htoxylin eosin(HE) staining was adopted to observe the pathological change in ileum of mice. Result:Compared with the normal group

the model group showed a significant increase in blood uric acid

remarkable decrease in jejunal and ileac ABCG2 mRNA and protein expressions

and notable rise in jejunal and ileac ABCG2 (P<0.05

P<0.01)

without any significant pathological change; after 2 weeks of administration with mangiferin

mangiferin (3.0

6.0 mg·kg-1) significantly lowered the serum uric acid compared with hyperuricemic mice; after administration with mangiferin (1.5

3.0

6.0 mg·kg-1) to hyperuricemic mice

mangiferin significantly increased the ABCG2 mRNA expression in jejunum and ileum; However

mangiferin (1.5

3.0

6.0 mg·kg-1) significantly caused the down-regulation of ABCG2 protein in jejunum and ileum (P<0.05). Conclusion:Mangiferin can obviously decrease the serum uric acid levels in hyperuricemic mice induced by urate

and restore ABCG2 mRNA and protein expressions of jejunum and ileum in hyperuricemic mice.