Objective: To explore the mechanism of Jinqi Jiangtang tablets on improving lipid metabolism disorder in type 2 diabetic rats from the perspective of metabonomics.Method: Type 2 diabetes mellitus model was established in 45 male Wistar rats fed with high fat diet and 3 times of intraperitoneal injection of streptozotocin(STZ

35 mg·kg-1).After successful modeling

the rats were randomly divided into normal group

model group and Jinqi Jiangtang tablets group.Jinqi Jiangtang tablets group was administered continuously for 4 weeks.At the end of administration

the blood was taken from the abdominal aorta and the blood lipid level was detected by a semi automatic biochemical analyzer

the pathological changes of the liver were observed by hematoxylin-eosin(HE) staining;the blood samples in different groups were detected by UPLC-Q-TOF-MS.Principal component analysis (PCA) and partial least squares-discriminant analysis(PLS-DA) were used to screen the differential metabolites among the normal group

model group and Jinqi Jiangtang tablets group

these differential metabolites were identified by mass spectrometry information and public database retrieval

and their metabolic pathways of the Kyoto encyclopedia of genes and genomes(KEGG) were analyzed.Result: Compared with the normal group

the level of blood lipid in the model group was significantly increased

the serum lipids in Jinqi Jiangtang tablets group were significantly lower than those in the model group.In the model group

obvious fatty granulocytes appeared in the liver

the hepatocyte fat granulocytes were significantly decreased in the administration group.There were 12 kinds of endogenous substances with significant changes in type 2 diabetes mellitus model group and the normal group.After intervention with Jinqi Jiangtang tablets

the levels of arachidonic acid

L-carnitine

lyso-phosphatidylcholine(LysoPC)(17:0)

LysoPC(P-18:0)

LysoPC[22:4(7Z

10Z

13Z

16Z)] and stearyl carnitine showed a different degree of callback.A key metabolic pathway

arachidonic acid pathway

was obtained by further analysis of the metabolic pathway.Conclusion: Jinqi Jiangtang tablets has a certain callback effect on the differential metabolites of lipid metabolism in rats with type 2 diabetes mellitus

and its mechanism is mainly related to arachidonic acid metabolic pathway.