Objective: To investigate the chemical constituents in Bushen Qiangshen pills (BQP) by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS). Method: The UPLC separation was performed on Agilent SB-C18 reversed-phase column (4.6 mm×100 mm

2.7 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 0.5 mL·min-1

and the column temperature was 40℃. Data were collected via positive and negative ion mode using ESI ion source. First of all

the qualitative analysis was conducted for the components with reference substances

and the major components without reference substances were identified according to the relative molecule weight and fragment information by UPLC-Q-TOF-MS as well as comparison with literature data. Result: Fifty compounds from BQP were identified

including 19 phenolic acids

25 flavonoids

4 olefins

1 phenylethanolate and 1 alkaloid. 12 components (protocatechuic acid

salidroside

protocatechuic aldehyde

chlorogenic acid

hyperin

isoquercitrin

specnuezhenide

epimedin C

icariin

kaempferide

baohuosideⅠ and astragalin) among them were qualitatively analyzed by comparing with reference substances. Conclusion: UPLC-Q-TOF-MS is convenient and rapid to analyze the chemical constituents in BQP

providing a reference for the study on material foundation of efficacy

quality control and clinical application of BQP.