Objective: To establish the method for simultaneous determination of the contents of tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

6

7

8

3'-pentamethoxy flavone based on high performance liquid chromatography diode array detection method of Calium verum. Method: Ultimate XB C18 column (4.6 mm×250 mm

5 μm) was used with methanol-0.05% phosphoric acid solution as the mobile phase for gradient elution

at a flow rate of 1.0 mL·min-1. The column temperature was 35℃. The determination wavelength was 260 nm for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

6

7

8

3'-pentamethoxy flavone. Result: There was a linear correlation within the range of 9.3-186 mg·L-1(r=0.999 4)

12.8-256 mg·L-1(r=0.999 3)

50-1 000 mg·L-1(r=0.999 3)

38.7-774 mg·L-1(r=0.999 5)

14.5-290 mg·L-1(r=0.999 2)

27.4-548 mg·L-1(r=0.999 7) and 8.6-172 mg·L-1(r=0.999 6) respectively for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin and 5

4'-dihydroxy-3

6

7

8

3'-pentamethoxy flavones. The average recovery rate was 98.15%(RSD 2.4%)

101.30%(RSD 2.2%)

99.18%(RSD 1.1%)

99.96%(RSD 1.4%)

101.88%(RSD 0.9%)

100.02%(RSD 2.3%) and 101.71%(RSD 1.4%)respectively for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

6

7

8

3'-pentamethoxy flavone. Conclusion: The analysis method is simple

rapid

reproducible

accurate

and reliable

and can be used to identify and determine the contents of seven constituents in G. verum

providing scientific basis for its quality control.