Objective: To establish a high performance liquid chromatography-mass spectrometry(HPLC-MS) method for simultaneously determining eight components in Xiaoer Anshen Bunao granule. Method: Agilent TC-C18(4.6 mm×250 mm

5 μm) column was adopted with acetonitrile-10 mol·L-1 ammonium acetate solution as the mobile phase for gradient elution

and the injection volume was 10 μL.Electrospray ionization (ESI) was used in both positive and negative modes

and the selected ion monitoring(SIM) were used for scanning. Result: Liquiritin

calycosin-7-O-β-D-glucoside

ammonium glycyrrhizinate

naringin

3

6'-disinapoyl sucrose

hesperidin

neohesperidin

astragaloside Ⅳ showed a good linearity in the ranges of 0.019 5-2.5 mg·L-1(r=0.999 1)

0.078 1-5 mg·L-1(r=0.999 2)

0.078 1-5 mg·L-1(r=0. 999 8)

0.390 6-50 mg·L-1(r=0.999 9)

0.019 5-2.5 mg·L-1(r=0.999 2)

0.078 1-10 mg·L-1(r=0.999 0)

0.312 5-20 mg·L-1(r=0.999 4)

0.019 5-1.25 mg·L-1(r=0.999 0)

respectively. Relative standard deviations (RSDs) of precision

stability and reproducibility were all less than 2.0%. The recoveries for the eight analytes were between 94.67% and 97.79%

with RSD less than 2.0%. The contents of liquiritin

calycosin-7-O-β-D-glucoside

ammonium glycyrrhizinate

naringin

3

6'-disinapoyl sucrose

hesperidin

neohesperidin

and astragaloside Ⅳ were 187.5-188.1

330.0-331.0

312.1-319.5

1 509.0-1 510.2

172.2-173.0

180.0-181.0

639.0-639.8

and 37.5-38.2 μg·g-1

respectively. Conclusion: This method is simple

accurate

sensitive and repeatable

which can be used for identification

quality evaluation of Xiaoer Anshen Bunao granule.