Objective: To study the chemical constituents from the ethyl acetate extract of Pteris vittata. Method: The whole plant of P. vittata was powdered and extracted with 95% EtOH for 3 times under reflux. After removal of the solvent

the 95% EtOH residue was suspended in water and successively extracted with petroleum ether

EtOAc and n-BuOH to obtain petroleum ether

EtOAc and n-BuOH extract respectively. The EtOAc extract was isolated and purified by silica gel

Sephadex LH-20

ODS columns and recrystallization

and the structures of their monomeric compounds were identified on the basis of physicochemical properties

NMR spectra

mass spectrum analysis and related literature. Result: Twelve compounds were isolated from the ethyl acetate extract and identified as apigenin(1)

luteolin(2)

luteolin-7

3'

4'-trimethyl ether(3)

chrysoeriol(4)

7-hydroxy-8-methoxycoumarin(5)

apigenin-7-O-β-D-glucuronide(6)

luteolin-7-O-β-D-glucopyranoside(7)

chrysoeriol-7-O-β-D-glucopyranoside(8)

astragalin(9)

p-hydroxybenzoic acid(10)

β-sitosterol(11) and β-daucosterol(12). Conclusion: Compounds 2-5 and 8-12 were firstly isolated from P. vittata

providing reference for its clinical application and pharmacological studies.