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纸质出版日期:2018
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黄涛, 安衍茹, 彭亮, 等. 外源激素处理对远志种子萌发及幼苗生长的影响[J]. 中国实验方剂学杂志, 2018,24(20):50-55.
HUANG Tao, AN Yan-ru, PENG Liang, et al. Effect of Exogenous Hormone Treatment on Germination and Seedling Growth of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 50-55.
黄涛, 安衍茹, 彭亮, 等. 外源激素处理对远志种子萌发及幼苗生长的影响[J]. 中国实验方剂学杂志, 2018,24(20):50-55. DOI: 10.13422/j.cnki.syfjx.20181919.
HUANG Tao, AN Yan-ru, PENG Liang, et al. Effect of Exogenous Hormone Treatment on Germination and Seedling Growth of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 50-55. DOI: 10.13422/j.cnki.syfjx.20181919.
目的:研究不同浓度吲哚乙酸(IAA),吲哚丁酸(IBA),6-苄氨基嘌呤(6-BA)和赤霉素(GA3)4种外源激素对药用植物远志种子萌发和幼苗生长的影响。方法:采用不同浓度的4种外源激素对远志种子进行适度浸泡处理,以蒸馏水为对照,测定其发芽势、发芽率、发芽指数和活力指数,发芽结束后测量株高、根长、鲜重及干重。结果:不同浓度GA3溶液处理与蒸馏水对照组相比对远志种子的萌发及幼苗生长均具有促进作用;10,20,30 mg·L-1 IAA,20,30 mg·L-1IBA和20,30 mg·L-16-BA对远志种子的萌发有不同程度的促进作用;当IAA,IBA,6-BA ≥ 40 mg·L-1及IBA,6-BA ≤ 10 mg·L-1时,对远志种子的发芽率有不同程度的抑制作用;对外源激素处理后的远志种子幼苗生长状况中,IAA,IBA和6-BA表现出不同程度的抑制。总体效果为GA3 > IAA > IBA > 6-BA。其中以150 mg·L-1GA3处理效果最好,发芽率、活力指数分别是对照组的1.17倍和2.13倍,并对种子的幼苗根伸长、幼苗鲜重增加等早期生长有明显影响,种子发芽整齐,出苗一致。结论:通过研究不同外源激素浸种对远志种子萌发及幼苗生长的影响,筛选出对其有促进作用的外源激素,可为远志的种苗培育、人工栽培提供一定的理论基础及数据参考。
Objective: To study the effect of different concentrations of IAA
IBA
6-BA and GA3 on the germination and seedling growth of medicinal plant Polygala tenuiflora.Method: P. tenuifolia seeds were treated with four exogenous hormones with different concentrations. The germination potential
germination rate
germination index and vigor index were measured with distilled water as control. After germination
the plant height
root length
fresh weight and dry weight were measured.Result: Compared with distilled water control group
different concentrations of GA3 solution had an effect in promoting the germination and seedling growth of P. tenuifolia; 10
20
30 mg·L-1 IAA
20
30 mg·L-1 IBA and 20
30 mg·L-16-BA had different effects in promoting the germination of P. tenuifolia. Under the conditions of IAA
IBA
6-BA ≥ 40 mg·L-1 and IBA
6-BA ≤ 10 mg·L-1
the germination rate of P. tenuifolia seeds was inhibited to varying extent. IAA
IBA and 6-BA showed different degrees of inhibition on the seedling growth of P. tenuifolia after exogenous hormone treatment. By the overall effect
the order was GA3 > IAA > IBA > 6-BA. Among them
treatment with 150 mg·L-1 GA3 had the best effect
the germination rate and vigor index were respectively 1.17 times and 2.13 times that of the control group
and had obvious effects on the early growth of seedlings
such as seedling root elongation and fresh weight increase.Conclusion: By studying the effects of different exogenous hormones soaking seeds on the seed germination and seedling growth of P. tenuifolia
the exogenous hormones with the promoting effect are screened out
in order to provide certain theoretical basis and data reference for the cultivation and artificial cultivation of P. tenuifolia.
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