南蛇藤提取物通过Cofilin1抑制胃癌BGC-823细胞转移的机制

王海波; 倪腾洋; 冯俊; 李丹; 金凤; 王维民; 朱光; 戴小军; 陶立德; 陶丽; 刘延庆

doi:10.13422/j.cnki.syfjx.20181921

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南蛇藤提取物通过Cofilin1抑制胃癌BGC-823细胞转移的机制

中医药对胃癌的实验研究专题 | 更新时间：2024-01-04

- 南蛇藤提取物通过Cofilin1抑制胃癌BGC-823细胞转移的机制
- Effect of Extract in Inhibiting Metastasis of Gastric Cancer BGC-823 Cells via Cofilin1
- 中国实验方剂学杂志 2018年24卷第19期页码：112-116
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81773944）;江苏省研究生科研创新计划项目（KYCX17_1892）;扬州大学优秀博士学位论文基金项目
- DOI：10.13422/j.cnki.syfjx.20181921
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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王海波, 倪腾洋, 冯俊, 等. 南蛇藤提取物通过Cofilin1抑制胃癌BGC-823细胞转移的机制[J]. 中国实验方剂学杂志, 2018,24(19):112-116.

WANG Hai-bo, NI Teng-yang, FENG Jun, et al. Effect of Extract in Inhibiting Metastasis of Gastric Cancer BGC-823 Cells via Cofilin1[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 112-116.
王海波, 倪腾洋, 冯俊, 等. 南蛇藤提取物通过Cofilin1抑制胃癌BGC-823细胞转移的机制[J]. 中国实验方剂学杂志, 2018,24(19):112-116. DOI： 10.13422/j.cnki.syfjx.20181921.

WANG Hai-bo, NI Teng-yang, FENG Jun, et al. Effect of Extract in Inhibiting Metastasis of Gastric Cancer BGC-823 Cells via Cofilin1[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 112-116. DOI： 10.13422/j.cnki.syfjx.20181921.

摘要

目的：探讨南蛇藤乙酸乙酯提取物（Celastrus orbiculatus extract，COE）抑制胃癌侵袭转移的作用及其机制。为胃癌的中医药预防与治疗寻求新的靶点，为开发有效的抗胃癌中药奠定理论和实验基础。方法：利用前期蛋白质二维电泳技术，在胃癌中筛选出COE作用于胃癌的差异大的靶点蛋白。然后利用蛋白免疫印迹法（Western blot）检测COE（20，40，80 mg·L-1）干预后细胞中丝切蛋白1（Cofilin 1，CFL1）水平的表达情况。最后利用分子克隆技术，将胃癌细胞株BGC-823中的CFL1表达抑制，然后联合COE进行干预，检测COE是否能影响胃癌细胞的侵袭转移。结果：在前期实验基础上经过细胞水平的验证，CFL1能够在胃癌细胞中稳定高表达；Western blot结果显示，COE能抑制胃癌细胞中CFL1的表达，经不同质量浓度COE（20，40，80 mg·L-1）处理24 h的BGC-823细胞，其CFL1的水平受到了不同程度的抑制。随着COE的浓度递增，CFL1的表达逐渐减弱。与空白组比较，COE40，80 mg·L-1组CFL1表达显著减少（P<0.01）；利用小干扰RNA（siRNA）技术干扰胃癌BGC-823细胞株后，CFL1表达受到明显抑制，与空白组比较，siRNA-CFL1慢病毒转染组BGC-823细胞中CFL1受到显著抑制（P<0.01）；细胞侵袭实验（transwell）显示COE能显著减少胃癌BGC-823细胞敲低表达后细胞的透膜细胞数（P<0.01）。结论：COE能明显抑制胃癌BGC-823细胞的转移，其机制可能与抑制CFL1的过度表达有关。

Abstract

Objective: To explore the role and mechanism of Celastrus orbiculatus extract (COE) in inhibiting the metastasis of gastric cancer

in order to seek new targets in preventing and treating gastric cancer with traditional Chinese medicine and lay a theoretical and experimental foundation for the development of effective anti-gastric cancer drugs are very important. Method: According to the results of the previous two-dimensional electrophoresis experiment

the differentially expressed target proteins of COE (20

80 mg·L-1) in gastric cancer were screened out. Then the expression of Cofilin 1 (CFL1) in the gastric cancer cells after COE intervention was detected by Western blot. Finally

the molecular cloning technique was used to inhibit the expression of CFL1 in gastric cancer BGC-823 cells

then COE was used to detect whether it could affect the metastasis of gastric cancer cells. Result: CFL1 was stably overexpressed in gastric cancer cells based on previous experiments. Western blot results showed that COE inhibited the expression of CFL1(P<0.01). Various concentrations of COE (20

80 mg·L-1) inhibited the expression of CFL1 in BGC-823 cells. And the expression of CFL1 was significantly inhibited by small interfering RNA (siRNA)(P<0.01). The transwell experiments showed that COE markedly reduced the number of transmembrane cells in low expressed BGC823 cells

with statistically significant differences(P<0.01). Conclusion: COE could significantly inhibit the metastasis of gastric cancer BGC-823 cells

and its mechanism may be related to the inhibition of overexpression of CFL1.

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