金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响

李丹; 金凤; 陶丽; 倪腾洋; 王海波; 冯俊; 朱光; 钱亚云; 丁岩冰; Masataka Sunagawa; 刘延庆

doi:10.13422/j.cnki.syfjx.20181922

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金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响

中医药对胃癌的实验研究专题 | 更新时间：2024-01-04

- 金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响
- Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells
- 中国实验方剂学杂志 2018年24卷第19期页码：117-123
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81773944）;江苏省自然科学基金项目（BK20141280）
- DOI：10.13422/j.cnki.syfjx.20181922
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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李丹, 金凤, 陶丽, 等. 金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响[J]. 中国实验方剂学杂志, 2018,24(19):117-123.

LI Dan, JIN Feng, TAO Li, et al. Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 117-123.
李丹, 金凤, 陶丽, 等. 金龙胶囊对胃癌细胞MGC-803和BGC-823侵袭转移能力的影响[J]. 中国实验方剂学杂志, 2018,24(19):117-123. DOI： 10.13422/j.cnki.syfjx.20181922.

LI Dan, JIN Feng, TAO Li, et al. Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 117-123. DOI： 10.13422/j.cnki.syfjx.20181922.

摘要

目的：探讨金龙胶囊对人胃癌MGC-803，BGC-823细胞侵袭和迁移能力的影响并对其具体机制进行探讨。方法：体外培养胃癌MGC-803，BGC-823细胞，分为金龙胶囊组（0.1，0.2，0.4，0.8 g·L-1），空白组和5-氟尿嘧啶（5-FU）组，采用噻唑蓝（MTT）比色法分别检测金龙胶囊对MGC-803，BGC-823细胞增殖的抑制作用，计算半数抑制率（IC50）；采用细胞侵袭（transwell）小室法和划痕实验检测金龙胶囊处理MGC-803和BGC-823细胞24 h后，细胞侵袭和迁移能力发生改变；蛋白免疫印迹法（Western blot）检测金龙胶囊处理MGC-803，BGC-823细胞24 h后E-钙黏素（E-cadherin），基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）蛋白表达情况。结果：金龙胶囊能明显抑制MGC-803，BGC-823细胞的增殖，呈一定的浓度及时间依赖性；与空白组比较，金龙胶囊能明细减少MGC-803，BGC-823细胞的穿膜细胞数目，可明显影响2种胃癌细胞的侵袭与迁移能力，并呈明显浓度依赖性（P<0.05）；金龙胶囊组的划痕距离较空白组明显降低，影响MGC-803，BGC-823细胞迁移，并呈浓度依赖性（P<0.05）；与空白组比较，金龙胶囊组能够提高MGC-803和BGC-823细胞中E-cadherin蛋白表达，降低MMP-2，MMP-9蛋白表达水平，且呈一定的量效关系（P<0.05）。结论：金龙胶囊能够抑制人胃癌MGC-803，BGC-823细胞的侵袭和迁移能力，其作用机制可能与上调E-cadherin表达，抑制MMP-2，MMP-9表达水平有关。本研究证实了金龙胶囊对胃癌MGC-803，BGC-823细胞抗侵袭转移的部分机制。

Abstract

Objective: To investigate the effect of Jinlong capsule(JLC) on the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells and its specific mechanism. Method: Gastric cancer MGC-803 and BGC-823 cells were cultured in vitro and divided into JLC group (with concentrations of 0.1

0.2

0.4

0.8 g·L-1)

negative control group and 5-fluorouracil (5FU) as positive control group. Methye thiazolye telrazlium (MTT) was used to detect the inhibitory effect of Jinlong capsule on the proliferation inhibition rate of MGC-803 and BGC-823 cells

in order to calculate the 50%inhibitory concentration (IC50); transwell and wound healing assay were used to detect the invasion and migration abilities of BGC-823 cell and MGC-803 cells after treatment with JLC for 24 h; after 24 h

the migration of MGC-803 and BGC-823 cells changed; Western blot method was used to detect the expression levels of E-cadherin

matrix metalloproteinase (MMP)-2 and MMP-9 after Jinlong capsule treatment for 24 h. Result: JLC could obviously inhibit the proliferation of MGC-803 and BGC-823 cells in a time-dependent manner; compared with the control group

JLC could significantly affect the invasion and migration abilities of the two kinds of gastric cancer cells in a concentration-dependent manner (P<0.05); the scratch distance of Jinlong capsule group was significantly lower than that in control group in a concentration-dependent manner (P<0.05); compared with control group

JLC group could increase the expression of E-cadherin and reduce the expression levels of MMP-2

MMP-9 in MGC-803 and BGC-823 cells

with a dose-response relationship (P<0.05). Conclusion: JLC can inhibit the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells

and its mechanism may be related to the up-regulation of the expression of E-cadherin and the inhibition of the expression levels of MMP-2

MMP-9.This study has confirmed part of mechanisms of JLC on the invasion and metastasis of gastric cancer MGC-803 and BGC-823 cells.

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