Objective: To investigate the effect of resveratrol in delaying senescence of human cardiac microvascular endothelial cells (HCMEC)

and to analyze its mechanism of action from the perspective of cytoskeleton. Method: Heat shock protein 27(HSP27) shRNA cell lines were established

and the cells were divided into 5 groups

the eighth generation replicative senescence model group (senescence group)

resveratrol group (10 μmol·L-1)

mock group

HSP27shRNA group

HSP27shRNA+resveratrol group (10 μmol·L-1). Cells senescence was detected by using β-galactosidase staining; cell counting kit-8 (CCK-8) was used to detect cell proliferation; laser confocal microscopy was used to observe the morphological changes of fibrous actin (F-actin) and globular actin (G-actin). Western blot was used to detect the protein expression of F-actin. Result: As compared with the senescence group

resveratrol could decrease the proportion of β-galactosidase stained (P<0.01)

promote cell proliferation(P<0.01)

maintain F-actin distribution in the cell periphery and G-actin distribution in the cell center

and reduce the protein expression(P<0.01). After silencing of heat shock protein 27 (HSP27)

as compared with mock group

HSP27shRNA group and HSP27shRNA+resveratrol group could increase the proportion of β-galactosidase stained(P<0.01)

decrease cell proliferation(P<0.01); F-actin around the cell periphery became irregular

sputtered and dissipated gradually

with unclear cell shape; G-actin showed blurred edges and stretched outside in cotton-like method

with staining dispersion in cell central region and irregular shape. The mean optical density value of F/G-actin was decreased(P<0.01)

and the protein expression was also decreased(P<0.01). Conclusion: Resveratrol could delay endothelial senescence

promote cell proliferation

and the mechanism may be associated with down-regulation of the F-actin expression by HSP27.