Objective: To explore the effect and mechanism of Bushen Tongluo decoction(BSTL) on the glucose metabolism of osteoporosis of ovariectomized rat model based on the expressions of peroxisome proliferator activated receptor γ coactivator-1α(PGC-1α) and glucose transporter 4(GLUT4) in fat and muscle.Method: Female rats were randomly divided into the sham operation group (n=22) and the operation group (n=60); and all of them underwent ovary resection for modeling. 70 days after surgery

10 rats from the two groups were randomly selected and put to death

and their right femurs were collected. The dual-energy X-ray bone density instrument was used to test whether the models were successful or not. After the successful modeling

the sham-operated group still had 12 rats

and the operation group still had 48 rats. The operation group was divided into model group

kidney-tonifying drugs group (5.4 g·kg-1)

deoppilant group (0.9 g·kg-1)

and BSTL group (6.3 g·kg-1)

with 12 rats in each group. The osteoporosis rat model was respectively given kidney-tonifying drugs

deoppilant

and BSTL by gavage for 70 days. Blood glucose and bone mineral density (BMD) of the rat model were tested after the experiment. The mRNA and protein expression levels of PGC-1α and GLUT4 were detected in adipose tissue and skeletal muscle by Real-time PCR and Western blot.Result: Compared with the sham-operated group

the blood glucose of model groups was raised. The expression levels of BMD

PGC-1α and GLUT4 in adipose tissue and skeletal muscle were obviously declined (P<0.01). Compare with model groups

the blood glucose level of BSTL was decreased (P<0.01)

and the expression levels of PGC-1α and GLUT4 were increased remarkably both in adipose tissue and skeletal muscle (P<0.05

P<0.01).Conclusion: BSTL increases the expression of GLUT4 and the level of glucose metabolism by promoting the expression of PGC-1α in adipose tissue and skeletal muscle.