秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子-B的影响

苏杉; 王蓉; 赵敏; 吴晨; 高慧琴

doi:10.13422/j.cnki.syfjx.20181933

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秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子-B的影响

药理 | 更新时间：2024-01-04

- 秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子-B的影响
- Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia
- 中国实验方剂学杂志 2018年24卷第19期页码：159-165
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金地区基金项目（81360648）
- DOI：10.13422/j.cnki.syfjx.20181933
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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苏杉, 王蓉, 赵敏, 等. 秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子-B的影响[J]. 中国实验方剂学杂志, 2018,24(19):159-165.

SU Shan, WANG Rong, ZHAO Min, et al. Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 159-165.
苏杉, 王蓉, 赵敏, 等. 秦艽不同配伍对风寒湿痹类风湿关节炎大鼠类风湿因子，C-反应蛋白及核转录因子-B的影响[J]. 中国实验方剂学杂志, 2018,24(19):159-165. DOI： 10.13422/j.cnki.syfjx.20181933.

SU Shan, WANG Rong, ZHAO Min, et al. Effect of Different Combined Administrations of Gentianae Macrophyllae Radix on Rheumatoid Factor, C-reactive Protein and Nuclear Factor-B in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 159-165. DOI： 10.13422/j.cnki.syfjx.20181933.

摘要

目的：观察秦艽不同配伍药对对风寒湿痹型类风湿性关节炎（rheumatoid arthritis，RA）模型大鼠血清类风湿因子（rheumatoid factor，RF），C-反应蛋白（C-reactive protein，CRP）及踝关节核转录因子-κB（nuclear factor kappa B，NF-κB）的影响。方法：选取80只SD大鼠并随机分为正常组，Ⅱ型胶原组，风寒湿病证模型组，雷公藤多苷组，单味秦艽组，秦威组（秦艽配伍威灵仙），秦桑组（秦艽配伍桑寄生），秦防组（秦艽配伍防己），每组10只。注射Ⅱ型胶原乳剂并施加风寒湿刺激制备风寒湿RA大鼠模型。模型建立完成后正常组，Ⅱ型胶原组，风寒湿病证模型组给予生理盐水灌胃，各给药组给予相应药液。实验过程中，每3 d测量一次大鼠左后足跖厚度，计算足跖肿胀度；实验第38天对各组大鼠进行关节炎指数（arthritis index，AI）评分；酶联免疫吸附测定法（ELISA）检测大鼠血清RF，CRP含量；蛋白免疫印迹技术检测踝关节NF-κB的表达；实时荧光定量聚合酶链式反应（Real-time PCR）技术检测踝关节NF-κB mRNA表达。结果：与正常组比较，Ⅱ型胶原组及风寒湿病证模型组足跖肿胀度，AI评分，血清RF，CRP水平，踝关节NF-κB蛋白表达水平及NF-κB mRNA水平显著升高（P<0.01）；与风寒湿病证模型组比较，各中药组足跖肿胀度，AI评分，血清RF，CRP水平，踝关节NF-κB蛋白表达水平及NF-κB mRNA水平均有所降低（P<0.05，P<0.01），其中秦威组降低最明显。结论：平温相配的秦威组对于风寒湿痹型类风湿关节炎的疗效优于平平相配的秦桑组、平寒相配的秦防组及单味秦艽组，实验结果与中医"疗寒以热药"的原则基本相符。

Abstract

Objective: To observe the effects of differences combined administrations of Gentianae Macrophyllae Radix (GMR) on serum rheumatoid factor (RF)

C-reactive protein (CRP) and ankle nuclear factor kappa B (NF-κB) in rheumatoid arthritis (RA) model rats with wind-cold-dampness arthralgia. Method: Eighty healthy SD rats were randomly divided into blank control group

collage Ⅱ model group

wind-cold-dampness syndrome model group

positive control group

single-taste GMR group

GMR-Clematidis Radix et Rhizoma(CRR) group (GC group)

GMR-Taxilli Herba group (GT group)

GMR-Stephaniae Tetrandrae Radix group (GS group)

with 10 rats in each group. Wind-cold-dampness Arthralgia model rats was induced in each group except for the blank group; collage Ⅱ model group was injected with collage Ⅱ and exposed to a wind-cold-dampness environment to prepare the wind-cold-dampness RA model of rats. After the establishment of the model

blank control group

collage Ⅱ model group and wind-cold-dampness syndrome model group were given normal saline

and the corresponding liquid medicines were given to each administration group. In the experiment

the thickness of the left posterior metatarsal of rats was measured every 3 days

and the swelling degree of the metatarsal was calculated. The arthritis index (AI) was evaluated on the 38th day of the experiment. The serum RF and CRP contents of rats were detected by enzyme linked immunosorbent assay (ELISA). The expression of NF-κB in ankle joint was detected by Western blot. The NF-κB mRNA expression in ankle joint was detected by Real-time fluorescence quantitative PCR. Result: Compared with the blank group

the swelling degree

AI score of foot

serum RF and CRP levels

of NF-κB protein and mRNA expressions in the ankle joint of collage Ⅱ model group and wind-cold-dampness syndrome model group were significantly increased (P<0.01). Compared with the wind-cold-dampness syndrome model group

the swelling degree

AI score of foot

serum RF and CRP levels

NF-κB protein and mRNA expressions in the ankle joint of the traditional Chinese medicine group were decreased(P<0.05

P<0.01)

especially in GC group. Conclusion: The therapeutic effect of mild and warm traditional Chinese medicine (TCM) is better than that of the combined administration of mild and cold TCM or mild TCM drugs for rheumatoid arthritis with wind-cold-dampness arthralgia. The experimental results basically conform to the principle of ‘treating cold diseases with hot medicine’ in TCM.

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