Objective: To explore the potential mechanism of Gegen Qinlian Tang(GQT) in the treatment of type 2 diabetes mellitus (T2DM) using network pharmacology. Method: Four chemical components of GQT and target genes of T2DM were collected in Computation Platform for Integrative Pharmacology of Traditional Chinese Medicine (TCMIP). The drug targets were screened out according to the Similarity of Two-Dimensional structures. The online Cytoscape technology in TCMIP was used to build the core target network and "herbs-ingredient-target-pathway" network. Gene Ontology(GO) gene function combined with Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment module was used to annotate and analyze the gene function and signaling pathway of the core target. Result: Based on the TCMIP

234 chemical components of GQT and 175 core targets were collected. Multi-dimensional network analysis showed that 68 active components had strong interactions with protein kinase C-δ(PRKCD)

glucokinase (GCK)

cytochrome C oxidase subunit (COX7C/COX5A/COX5B)

ATP synthase F1 subunit (ATP5F1A/ATP5F1C)

insulin receptor (INSRS) and other 22 major proteins; the biological pathways mainly involved in the endocrine system

cell growth and death

FoxO signaling pathway

adipocytokine signaling pathway

insulin signaling pathway and so on. Conclusion: The findings preliminarily reveal the effective mechanism of GQT in treating T2DM

which is related to anti-inflammation

anti-oxidation stress

anti-insulin β cell injury

and improvement of glycolipid metabolism and complications.