Objective:To screen and optimize the proper proportion of Ruditapes philippinarum(RP) and Schisandra chinensis fructus(SCF)

and investigate its protective effect on liver injury in mice. Method:Taking activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in mice serum as indexes

uniform design was applied to investigate protective effect of RP and SCF with different proportion on liver injury mice induced by CCl4.The optimum proportion of RP to SCF was selected according to curve fitted by SAS 9.3 software.Validation of optimized proportion was implemented on the model of both CCl4 and alcohol induced mice liver injury at various dose levels. Result:Compatibility group of RP and SCF could significantly decrease the activities of AST and ALT in mice serum.The results of regression analysis showed that the activities of ALT and AST decreased with increasing of dose level of RP

and first decreased and then increased with increasing of dose level of SCF;however

there was no interactive effect between dose levels of RP and SCF.The optimum compatibility doses of RP and SCF were 0.57

0.30 g·kg-1

namely

the ratio of SCF to RP was 1:1.9.The validation experiment also showed that the compatibility group was better than the single group at the optimum dose. Conclusion:The compatibility of RP and SCF can protect liver.The study provides experimental basis for the development and utilization of RP resources.