Objective: Taking couplet medicines of Psoraleae Fructus and Notoginseng Radix et Rhizoma as an example

the mechanism of tonifying kidney and promoting blood circulation in treatment of osteoporosis was explored from the perspective of integrated pharmacology. Method: Based on the Integrated Pharmacology Platform(TCMIP)

the chemical constituents of Psoraleae Fructus and Notoginseng Radix et Rhizoma were collected

and all targets of osteoporosis were obtained by taking "osteoporosis" as a key word of disease.Through the protein-protein interaction(PPI) database in TCMIP

the PPI between the targets of Psoraleae Fructus-Notoginseng Radix et Rhizoma and the targets of osteoporosis was obtained

a key target network between drug pairs and diseases was established

and the target genes

protein molecular function

intracellular localization

and the biological reactions and pathways were determined based on gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) database. Result: Psoraleae Fructus and Notoginseng Radix et Rhizoma had a total of 34 targets.The mechanism may be related to biological processes

such as phosphorylation of Smad protein

introduction of Smad protein into the nucleus

vitamin D reaction element binding

etc;and it was associated with osteoclast differentiation

oxidative phosphorylation

peroxisome proliferator-activated receptor signaling pathway

endocrine and other factors regulating calcium resorption and other biological pathways. Conclusion: Psoraleae Fructus and Notoginseng Radix et Rhizoma can participate in the regulation of Smad protein and its signal transduction process.It can provide some ideas for tonifying kidney and promoting blood circulation preparations to achieve the prevention and treatment of osteoporosis by regulating the Smad protein

thereby affecting Runt related transcription factor 2 and other transcription factors

and extracellular signal-regulated kinase and other signal pathways.