Objective:To study the effect of ginsenoside Rg5(12.5

25

50 μmol·L-1) on the invasion and migration and the expressions of microRNA-125b(miR-125b)

StAR-related lipid transfer domain protein 13(STARD13) and neuraminidase 1 (NEU1)in gastric cancer BGC-823 cell

in order to investigate the possible mechanism of ginsenoside Rg5 in inhibiting the invasion and migration of gastric cancer. Method:Transwell was used to detect the invasion and migration of gastric cancer BGC-823 cell

Real-time PCR was used to detect the mRNA expressions of miR-125b

STARD13 and NEU1

and Western blot was used to detect the protein expressions of miR-125b

STARD13 and NEU1. Result:Middle and high-dose ginsenoside Rg5 could effectively inhibit the invasion and migration of BGC-823 cells. Compared with the blank group

the relative expression of miR-125b in low-dose group was decreased

the relative expressions of STARD13 and NEU1 mRNA in the low-dose group were increased; the relative expression of miR-125b in middle and high-dose groups was decreased

the relative expressions of STARD13 and NEU1 mRNA in middle and high-dose groups were increased(P<0.01). Compared with the blank group

the relative expression of STARD13 protein in low and middle-dose groups were increased

and NEU1 protein in low-dose group was increased; the relative expression of STARD13 protein in high-dose group was increased(P<0.05)

and NEU1 protein in middle and high-dose groups was increased(P<0.01). Conclusion:Ginsenoside Rg5 may inhibit the invasion and migration of gastric cancer BGC-823 cells by decreasing the expression of miR-125b and up-regulating the expressions of miR-125b target genes STARD13 and NEU1.