车前子水煎液对痛风性肾病大鼠的肾保护作用及机制

赵宏; 柴桂芳; 刘颖斐; 李洋; 肖洪彬; 匡海学

doi:10.13422/j.cnki.syfjx.20182035

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车前子水煎液对痛风性肾病大鼠的肾保护作用及机制

药理 | 更新时间：2024-01-04

- 车前子水煎液对痛风性肾病大鼠的肾保护作用及机制
- Renal Protective Effect and Preliminary Mechanism of Water Decoction from Plantaginis Semen on Rats with Gouty Nephropathy
- 中国实验方剂学杂志 2018年24卷第20期页码：108-114
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重点基础研究发展计划（973计划）项目（2013CB531801）;中国博士后科学基金面上项目（2015M571454）;黑龙江省博士后科学基金面上项目（LBH-Z14204）;黑龙江省科学基金青年项目（QC2018119）;黑龙江省教育厅青年创新人才项目（UNPYSCT-2016095）;佳木斯大学青年创新人才培养计划项目（22Zq201501）
- DOI：10.13422/j.cnki.syfjx.20182035
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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赵宏, 柴桂芳, 刘颖斐, 等. 车前子水煎液对痛风性肾病大鼠的肾保护作用及机制[J]. 中国实验方剂学杂志, 2018,24(20):108-114.

ZHAO Hong, CHAI Gui-fang, LIU Ying-fei, et al. Renal Protective Effect and Preliminary Mechanism of Water Decoction from Plantaginis Semen on Rats with Gouty Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 108-114.
赵宏, 柴桂芳, 刘颖斐, 等. 车前子水煎液对痛风性肾病大鼠的肾保护作用及机制[J]. 中国实验方剂学杂志, 2018,24(20):108-114. DOI： 10.13422/j.cnki.syfjx.20182035.

ZHAO Hong, CHAI Gui-fang, LIU Ying-fei, et al. Renal Protective Effect and Preliminary Mechanism of Water Decoction from Plantaginis Semen on Rats with Gouty Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 108-114. DOI： 10.13422/j.cnki.syfjx.20182035.

摘要

目的：观察车前子水煎液对痛风性肾病大鼠的药效学指标及对肾组织半胱氨酸蛋白酶-1（cysteine-aspartic acid protease-1，Caspase-1），Nod样受体蛋白3（Nod-like receptor protein 3，NLRP3）和凋亡相关点样蛋白（fapoptosis-associated speck-like protein，ASC）表达的影响，探讨车前子水煎液对痛风性肾病大鼠的肾保护作用和初步机制。方法：60只SD大鼠随机分为6组，分别为正常组，模型组，阳性药组（别嘌醇，50 mg·kg-1），车前子水煎液高、中、低剂量组（1.62，0.81，0.27 g·kg-1），除正常组外，其余各组采用腺嘌呤和酵母联用的方法制作痛风性肾病大鼠模型，给予车前子水煎液，观察肾脏特征；计算肾重指数；检测大鼠血清中尿酸（uric acid，UA），尿素氮（blood urea nitrogen，BUN），肌酐（creatinine，SCr），丙氨酸氨基转移酶（cereal third transaminase，ALT），天门冬氨酸氨基转移酶（aspartate transaminase，AST），碱性磷酸酶（alkalinephosphatase，ALP）；采用光镜观察肾脏病理形态学变化；蛋白免疫印迹法（Western bolt）和免疫组化法（immunohistochemical methods）测定肾组织中NLRP3，ASC，Caspase-1蛋白表达量。结果：与模型组比较，别嘌醇组和车前子水煎液各剂量组的肾脏体积减小，别嘌醇组和车前子中剂量组肾重指数降低，车前子高剂量组肾重指数降低明显，别嘌醇组和车前子水煎液各剂量组血清UA，BUN，SCr，ALT，AST，ALP明显降低（P<0.05，P<0.01），肾组织病变程度均有一定减轻，其中车前子高、中剂量组最好，别嘌醇组和车前子水煎液高剂量组中NLRP3，ASC和Caspase-1蛋白的表达明显降低（P<0.05，P<0.01），车前子水煎液中剂量组中NLRP3蛋白的表达降低（P<0.05）。结论：车前子对痛风性肾病大鼠具有较好肾保护作用，可能与其下调肾组织中NLRP3，ASC和Caspase-1蛋白表达，抑制下游炎性细胞因子释放有关。

Abstract

Objective: To observe pharmacodynamics index and effect of water decoction from Plantaginis Semen on Nod-like receptor protein 3 (NLRP3)

fapoptosis-associated speck-like protein (ASC) and cysteine-aspartic acid protease-1 (Caspase-1) of kidneys of rats with gouty nephropathy (GN)

in order to investigate its renal protective effect and preliminary mechanism.Method: Adenine and yeast were used to make rat models of GN. The therapeutic effect and mechanism of water decoction from Plantaginis Semen on GN were studied by detecting the kidney characteristics and weight index; the levels of serum uric acid (UA)

blood urea nitrogen (BUN)

creatinine (SCr)

cereal third transaminase (ALT)

aspartate transaminase (AST)

alkalinephosphatase (ALP); renal histopathological reduced under light microscope; the expressions of NLRP3

ASC

Caspase-1 were detected by Western blot and immunohistochemical methods.Result: Compared with the model group

the volumes of kidneys in allopurinol group and water decoction from Plantaginis Semen groups were smaller; the kidney weight index in allopurinol group and middle-dose water decoction from Plantaginis Semen group were lower

and the figure was significantly lower in high-dose group; the expression levels of UA

BUN

SCr

ALT

AST

ALP in serum in allopurinol group and water decoction from Plantaginis Semen groups were lower (P<0.05

P<0.01)

and the pathological changes in renal tissues in GN model rats were relieved

especially in high and middle-dose water decoction from Plantaginis Semen groups. Compared with the model group

the protein expression levels of NLRP3

ASC and Caspase-1 in kidney tissues in allopurinol group and high-dose water decoction from Plantaginis Semen group were down-regulated(P<0.05

P<0.01)

only the protein expression level of NLRP3 was down-regulated in middle-dose group(P<0.05).Conclusion: Water decoction from Plantaginis Semen may protect rats with GN by down-regulating the protein expression levels of NLRP3

ASC

Caspase-1 and inhibiting release of downstream inflammatory factors.

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