Objective:To study the chemical constituents from Guizhou Miao medicine Reineckia carnea

and provide a scientific basis for explaining the pharmacological activities of R. carnea and its further development and utilization. Method:The 20 kg air-dried whole plant of R. carnea was extracted with 80% EtOH for three times by heating reflux

2

2 and 1 h respectively. These 3 extracts were combined

filtered

decompressed and concentrated into extract

which was then dissolved in water. Then the concentrated residue was successively partitioned with petroleum ether

EtOAc and n-BuOH to obtain 28 g petroleum ether fraction

305 g EtOAc fraction and 812 g n-BuOH fraction. The chemical constituents of EtOAc and n-BuOH fractions were isolated and purified by repeated silica gel column chromatography

macroporous resin

MCI

ODS

Sephadex LH-20 gel column chromatography and Pre-HPLC

and their structures were identified on the basis of physical and chemical properties

HR-ESI-MS

NMR spectral analysis and literature data. Result:Thirteen compounds were isolated and identified as 2α

3β-dihydroxy-5α-pregn-16-en-20-one(1)

(25R)-26-hydroxycholesterol (2)

diosgenin (3)

β-sitosterol (4)

β-daucosterol (5)

isorhamnetin-3-O-rutinoside (6)

farrerol (7)

luteolin (8)

1

2

8-trihdroxy-5

6-dimethoxyxanthone (9)

swertiamarin (10)

di (2-ethylhexyl) phthalate (11)

alternariol monomethyl ether (12)

and N-trans-feruloyltyramine (13). Conclusion:Compounds 1

2

6-10

12

and 13 were isolated from this plant for the first time.