乌梅丸对2型糖尿病模型大鼠NF-B p65及GLP-1的影响

吴帆; 刘圣徽; 朱金华; 周国佩; 万红娇

doi:10.13422/j.cnki.syfjx.20182142

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乌梅丸对2型糖尿病模型大鼠NF-B p65及GLP-1的影响

药理 | 更新时间：2024-09-23

- 乌梅丸对2型糖尿病模型大鼠NF-B p65及GLP-1的影响
- Effect of Wumeiwan on NF-B p65 and GLP-1 in Type 2 Diabetes Mellitus
- 中国实验方剂学杂志 2018年24卷第21期页码：144-148
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省教育厅科学技术研究项目（GJJ170737）;2018年度江西中医药大学校级创新基金项目（JZYC18S33）
- DOI：10.13422/j.cnki.syfjx.20182142
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
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吴帆, 刘圣徽, 朱金华, 等. 乌梅丸对2型糖尿病模型大鼠NF-B p65及GLP-1的影响[J]. 中国实验方剂学杂志, 2018,24(21):144-148.

WU Fan, LIU Sheng-hui, ZHU Jin-hua, et al. Effect of Wumeiwan on NF-B p65 and GLP-1 in Type 2 Diabetes Mellitus[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(21): 144-148.
吴帆, 刘圣徽, 朱金华, 等. 乌梅丸对2型糖尿病模型大鼠NF-B p65及GLP-1的影响[J]. 中国实验方剂学杂志, 2018,24(21):144-148. DOI： 10.13422/j.cnki.syfjx.20182142.

WU Fan, LIU Sheng-hui, ZHU Jin-hua, et al. Effect of Wumeiwan on NF-B p65 and GLP-1 in Type 2 Diabetes Mellitus[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(21): 144-148. DOI： 10.13422/j.cnki.syfjx.20182142.

摘要

目的：研究乌梅丸对2型糖尿病模型大鼠血糖及其核转录因子-κB（nuclear factor-κB，NF-κB）和胰高血糖素样肽-1（glucagon-like peptide-1，GLP-1）蛋白表达的影响。方法：将60只大鼠随机选取10只为正常组，余50只以高糖高脂乳剂灌胃8周后联合链脲佐菌素（STZ）腹腔注射诱导2型糖尿病模型，剔除造模未成功大鼠，纳入30只糖尿病模型大鼠，随机平均分成模型组、二甲双胍组、乌梅丸组。正常组和模型组分别灌胃（ig）生理盐水20 mL·kg-1·d-1，二甲双胍组ig二甲双胍200 mg·kg-1·d-1，乌梅丸组ig乌梅丸生药20 g·kg-1·d-1，28 d后监测大鼠血糖，采用蛋白免疫印迹法（Western blot）检测结肠组织NF-κB p65，GLP-1蛋白表达，免疫组化法检测胰腺组织NF-κB p65，GLP-1蛋白表达水平。结果：与正常组比较，模型组大鼠空腹血糖明显升高，结肠和胰腺组织NF-κB p65蛋白表达量明显上升（P<0.01），GLP-1蛋白表达量下降（P<0.05）；与模型组比较，乌梅丸组空腹血糖均明显降低（P<0.01），结肠和胰腺组织NF-κB p65蛋白表达量明显降低（P<0.01），GLP-1蛋白表达量明显上升（P<0.01）。结论：乌梅丸可以通过调节2型糖尿病模型大鼠空腹血糖，降低NF-κB的表达，上调GLP-1的表达，从而达到防治2型糖尿病的作用。

Abstract

Objective: To study the effect of Wumeiwan on blood glucose

nuclear factor-κB (NF-κB) and glucagon-like peptide-1(GLP-1) protein expression in rats with type 2 diabetes mellitus. Method: A total of 60 rats were randomly divided into normal group (n=10)

and the remaining 50 rats received intragastric administration of a high-sugar

high-fat emulsion for 8 weeks and then combined with the intraperitoneal injection of streptozotocin (STZ) to induce type 2 diabetes mellitus. The unsuccessful modeling rats were eliminated. A total of 30 diabetic rats were selected

and randomly divided into model group

metformin group and Wumeiwan group. The normal group and the model group received 20 mL·kg-1·d-1normal saline by intragastric administration respectively. 200 mg·kg-1·d-1Metformin crude drug and Wumeiwan crude drug were respectively given in Metformin group and Wumeiwan group.The blood glucose was monitored after 28 d. The protein expression levels of NF-κB p65 and GLP-1 in colon tissues were detected by Western blot. The immunohistochemical method was used to detect the expression levels of NF-κB p65 and GLP-1 in pancreatic tissue. Result: As compared with the normal group

the fasting blood glucose of rats in the model group was significantly increased

and the protein expression levels of NF-κB p65 in the colon and pancreas tissues were significantly increased (P<0.01)

while the protein expression levels of GLP-1 were decreased (P<0.05). As compared with model group

the fasting blood glucose of Wumeiwan group was significantly decreased (P<0.01)

and the protein expression levels of NF-κB p65 protein in the colon and pancreas were significantly reduced (P<0.01)

while the protein expression levels of GLP-1 were increased (P<0.01). Conclusion: Wumeiwan can regulate the fasting blood glucose of rats

reduce the expression of NF-κB and increase the expression of GLP-1

thus achieving the prevention and treatment effect on type 2 diabetes.

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