肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响

王启新; 陈则华; 罗琥捷; 卢文吉; 李春; 屠鹏飞

doi:10.13422/j.cnki.syfjx.20182217

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肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响

药理 | 更新时间：2024-09-23

- 肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响
- Effect of Different Fractions of Cistanches Herba Extracting in Improving Sexual Ability of Kidney-Yang Deficiency Rats
- 中国实验方剂学杂志 2018年24卷第22期页码：95-101
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81673802）
- DOI：10.13422/j.cnki.syfjx.20182217
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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王启新, 陈则华, 罗琥捷, 等. 肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响[J]. 中国实验方剂学杂志, 2018,24(22):95-101.

WANG Qi-xin, CHEN Ze-hua, LUO Hu-jie, et al. Effect of Different Fractions of Cistanches Herba Extracting in Improving Sexual Ability of Kidney-Yang Deficiency Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 95-101.
王启新, 陈则华, 罗琥捷, 等. 肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响[J]. 中国实验方剂学杂志, 2018,24(22):95-101. DOI： 10.13422/j.cnki.syfjx.20182217.

WANG Qi-xin, CHEN Ze-hua, LUO Hu-jie, et al. Effect of Different Fractions of Cistanches Herba Extracting in Improving Sexual Ability of Kidney-Yang Deficiency Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 95-101. DOI： 10.13422/j.cnki.syfjx.20182217.

摘要

目的：观察肉苁蓉不同提取部位对氢化可的松致肾阳虚模型大鼠性能力的影响。方法：将50只雄性SD大鼠随机分为正常组、阳虚模型组、肉苁蓉总寡糖组、肉苁蓉总多糖组、肉苁蓉苯乙醇总苷组。连续14 d肌肉注射氢化可的松制备肾阳虚动物模型，给药组按肉苁蓉生药量2.3 g·kg-1对应给予肉苁蓉总寡糖103.82 mg·kg-1，肉苁蓉总多糖115.64 mg·kg-1，肉苁蓉苯乙醇总苷201.48 mg·kg-1水溶液，每日1次。14 d后测定各组大鼠的勃起潜伏期，交配能力各指标（包括捕捉潜伏期、捕捉次数、射精潜伏期、射精次数、捕捉率、射精率等），脏器湿重及精浆果糖含量，血浆环磷酸腺苷（cAMP）/环磷酸乌苷（cGMP）值，血清激素睾酮（T），雌二醇（E2），促卵泡生成素（FSH），促黄体生成素（LH）及促性腺激素释放激素（GnRH）水平，观察睾丸病理形态变化。结果：肉苁蓉不同提取部位对肾阳虚大鼠的勃起功能、交配能力、脏器湿重及精浆果糖含量，血浆cAMP/cGMP值，血清激素水平，睾丸病理形态变化均有作用。其中肉苁蓉苯乙醇总苷提取物能够显著缩短大鼠的勃起潜伏期（P<0.05）及捕捉潜伏期（P<0.01），显著增加捕捉次数（P<0.01），睾丸（P<0.05），精囊腺（P<0.01）湿重及精浆果糖含量（P<0.05），显著升高血浆cAMP/cGMP值（P<0.01）及血清T（P<0.01），FHS（P<0.01），GnRH（P<0.05）水平，显著降低血清E2（P<0.05），LH（P<0.05）水平，显著改善睾丸病理形态。结论：肉苁蓉不同提取部位可通过调控氢化可的松致肾阳虚大鼠模型的激素水平不同程度提高其性能力，其中苯乙醇总苷可显著改善肾阳虚大鼠性能力相关指标。

Abstract

Objective:To observe the effect of extracts from on the sexual performance of rats with kidney-Yang deficiency induced by hydrocortisone. Method:Fifty male SD rats were randomly divided into normal control group

kidney-Yang deficiency model group

Cistanches Herba total oligosaccharide group

Cistanches Herba total polysaccharide group

and Cistanches Herba total phenylethanol glycosides

with 10 in each group. The animal model of kidney-Yang deficiency was prepared through intramuscular injection with hydrocortisone for 14 consecutive days. The medication groups were given aqueous solutions of total oligosaccharides of Cistanches Herba(103.28 mg·kg-1)

total polysaccharides of Cistanches Herba (115.64 mg·kg-1)

and total glycosides of phenylethanol of Cistanches Herba (201.48 mg·kg-1)

according to the dose of 2.3 g·kg-1 of Cistanches Herba raw material once a day. After 14 days

erectile latency

indexes of mating ability (including capture latency

the number of captures

the ejaculation latency

the number of ejaculations)

organ wet weight and berry sugar content

plasma cyclic adenosine monophosphate(cAMP)/cyclic guanosine monophosphate(cGMP) ratio

serum hormone testosterone (T)

estradiol (E2)

and follicle-stimulating hormone (FSH)

luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels were determined in each group of rats

and the testicular morphological changes were observed. Result:The extracts from different fractions of Cistanches Herba had the effect on erectile function

mating ability

organ wet weight and berry sugar content

plasma cAMP/cGMP value

serum hormone level

and testicular pathological changes in rats with kidney-Yang deficiency. Particularly

the extracts of total phenylethanol glycosides had the best effect

and could significantly reduce the erectile latency (P<0.05) and capture latency (P<0.01)

significantly increase the times of capture (P<0.01)

testis (P<0.05)

seminal vesicles (P<0.01) wet weight and berry sugar content (P<0.05)

significantly increase plasma cAMP/cGMP values (P<0.01)

serum T (P<0.01)

FHS (P<0.01)

GnRH (P<0.05) levels

significantly reduce serum E2 (P<0.05)

LH (P<0.05) levels

and significantly improve pathological morphology of testis in rats. Conclusion:Extracts from different fractions of Cistanches Herba can regulate hormone levels in rats with kidney-Yang deficiency induced by hydrocortisone and further improve their sexual ability. The total glycosides of phenylethanol can significantly improve the indexes related to sexual performance in rats with kidney-Yang deficiency.

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